Hypocrellin B-mediated sonodynamic action induces apoptosis of hepatocellular carcinoma cells

  • Ultrasonics. 2012 Apr;52(4):543-6. doi: 10.1016/j.ultras.2011.11.007.
Xinna Wang  1 ,  Albert Wingnang Leung ,  Yuan Jiang ,  Heping Yu ,  Xiaohong Li ,  Chuanshan Xu
Affiliations
  • 1. Department of Ultrasound Medicine, The Affiliated Hospital, Xi'an Medical University, Xi'an, China.
PMID: 22172458 DOI: 10.1016/j.ultras.2011.11.007
Abstract

Objective: The present study aims to investigate Apoptosis of hepatocellular carcinoma cells induced by hypocrellin B-mediated sonodynamic action.

Methods: The hypocrellin B concentration was kept constant at 2.5 μM and cells from the hepatocellular carcinoma HepG2 cell line were exposed to ultrasound with an intensity of 0.46 W/cm(2) for 8s. Cell cytotoxicity was quantified using an MTT assay 24 h after sonodynamic therapy (SDT) of hypocrellin B. Apoptosis was investigated using a flow cytometry with Annexin V-FITC and propidium iodine staining. Intracellular Reactive Oxygen Species (ROS) levels were detected using a flow cytometry with 2,7-dichlorodihydrofluorecein diacetate (DCFH-DA) staining.

Results: The cytotoxicity of hypocrellin B-mediated sonodynamic action on HepG2 cells was significantly higher than those of Other treatments including ultrasound alone, hypocrellin B alone and sham treatment. Flow cytometry showed that hypocrellin B-induced sonodynamic action markedly enhanced the apoptotic rate of HepG2 cells. Increased ROS was observed in HepG2 cells after being treated with hypocrellin B-mediated sonodynamic action.

Conclusions: Our data demonstrated that hypocrellin B-mediated sonodynamic action remarkably induced Apoptosis of HepG2 cells, suggesting that Apoptosis is an important mechanism of cell death induced by hypocrellin B-mediated SDT.

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