Sesquiterpenoids from Incarvillea arguta: absolute configuration and biological evaluation

  • J Nat Prod. 2012 Jun 22;75(6):1025-9. doi: 10.1021/np200912p.
Yong-Ming Yan  1 ,  Gui-Sheng Wu ,  Xiao-Ping Dong ,  Lan Shen ,  Yan Li ,  Jia Su ,  Huai-Rong Luo ,  Hua-Jie Zhu ,  Yong-Xian Cheng
Affiliations
  • 1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, People's Republic of China.
PMID: 22620677 DOI: 10.1021/np200912p
Abstract

Diincarvilones A-D (1-4), incarvilone A (5), and a known compound, argutosine B (6), were isolated from Incarvillea arguta. The structures, including the absolute configurations of the new compounds, were determined by NMR spectroscopy, X-ray diffraction analysis, CD spectroscopy, and a variety of computational methods. The biological properties of these substances, including effects on intracellular CA(2+) influx, nitric oxide (NO) production, and human Cancer cells, were evaluated. The results showed that at the concentration of 10 μM (in HBSS buffer) diincarvilones A and B cause a persistent increase in cytoplasmic calcium levels in A549 cells.