Accelerated polymer-polymer click conjugation by freeze-thaw treatment

  • Bioconjug Chem. 2012 Aug 15;23(8):1503-6. doi: 10.1021/bc300182y.
Hiroyasu Takemoto  1 ,  Kanjiro Miyata ,  Takehiko Ishii ,  Shota Hattori ,  Shigehito Osawa ,  Nobuhiro Nishiyama ,  Kazunori Kataoka
Affiliations
  • 1. Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-8656, Japan.
PMID: 22746327 DOI: 10.1021/bc300182y
Abstract

Herein, we report a unique technique to accelerate polymer-SNA conjugation based on copper-free click chemistry: gradual freeze-thawing of the reaction solution substantially increases the conjugation rate possibly because of the reactant concentration at the microenvironment scale. This technique was applied to the conjugation between a small interfering RNA (siRNA) and PEG in an aqueous buffer at/below room temperature.

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