Structural characterization of minor metabolites and pharmacokinetics of ganoderic acid C2 in rat plasma by HPLC coupled with electrospray ionization tandem mass spectrometry

  • J Pharm Biomed Anal. 2013 Mar 5;75:64-73. doi: 10.1016/j.jpba.2012.11.024.
Xiao-Yu Guo  1 ,  Dan Liu ,  Min Ye ,  Jian Han ,  Sa Deng ,  Xiao-Chi Ma ,  YanYan Zhao ,  Baojing Zhang ,  Xuan Shen ,  Qing-Ming Che
Affiliations
  • 1. Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
PMID: 23312386 DOI: 10.1016/j.jpba.2012.11.024
Abstract

The metabolites and pharmacokinetics of ganoderic acid C2 (GAC2), a bioactive triterpenoid in Ganoderma lucidum in rat plasma were investigated by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Totally, ten minor phase I metabolites of GAC2 were characterized after oral administration of GAC2, on the basis of their mass fragmentation pathways or direct comparison with authentic compounds by high-performance liquid chromatography coupled with diode array detection and electrospray ion trap tandem mass spectrometry (HPLC-DAD-ESI-MS(n)), and liquid chromatography coupled with electrospray ionization hybrid ion trap and time-of-flight mass spectrometry (LC-ESI-IT-TOF/MS) methods. Moreover, a rapid and specific method for quantification of GAC2 in rat plasma after oral administration was developed by using a liquid-liquid extraction procedure and HPLC-ESI-MS/MS analysis. It is the first time to report the metabolites and pharmacokinetics of GAC2.

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