Effects of d-menthol stress on the growth of and microcystin release by the freshwater cyanobacterium Microcystis aeruginosa FACHB-905

  • Chemosphere. 2014 Oct;113:30-5. doi: 10.1016/j.chemosphere.2014.03.084.
Xi Hu  1 ,  Yunguo Liu  2 ,  Guangming Zeng  3 ,  Weihua Xu  3 ,  Xinjiang Hu  1 ,  Zhilin Zhu  4 ,  Pingyang Zhang  1 ,  Yaqin Wang  1
Affiliations
  • 1. College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China.
  • 2. College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, PR China. Electronic address: [email protected].
  • 3. College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, PR China.
  • 4. Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, PR China.
PMID: 25065786 DOI: 10.1016/j.chemosphere.2014.03.084
Abstract

The effects of d-menthol on the growth of Microcystis aeruginosa FACHB-905 and microcystin (MCY) concentration were evaluated by batch culture experiments. The algal biomass and the intracellular and extracellular MCY concentrations were evaluated during 5d incubation. After the d-menthol exposure, the dry weight of the cells gradually decreased; the decrease in the dry weight after 5d exposure was 29, 12, and 2mgL(-1) when the initial cell densities were 1.4×10(7), 1.2×10(6), and 2.9×10(5)cellmL(-1), respectively. The results indicate that the d-menthol exposure inhibited the cellular growth, thus also inhibiting the increase of the total MCY concentration. In the presence of d-menthol, the intracellular MCY was gradually released into the medium after the Cell Lysis. The extracellular MCY concentration in the medium was significantly higher in the d-menthol-exposed samples than in the control samples, confirming that d-menthol cannot decompose the extracellular MCY.

Keywords
Microcystin; Microcystis aeruginosa; Protein pattern; d-Menthol.
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