Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine

  • Talanta. 2014 Dec;130:536-41. doi: 10.1016/j.talanta.2014.07.044.
Yi Cui  1 ,  Jie Yu  2 ,  Suling Feng  1
Affiliations
  • 1. School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
  • 2. Department of Chemistry, Xinxiang Medical University, Xinxiang 453007, China.
PMID: 25159443 DOI: 10.1016/j.talanta.2014.07.044
Abstract

A novel fluorescence method for the determination of guanine was developed based on the fluorescence enhancement of Cu(2+)-nuclear fast red complex in the presence of guanine in Tris-HCl buffer. The complex of Cu(2+) with nuclear fast red resulted in a dramatic quenching of the fluorescence intensity. Nuclear fast red were dissociated from the complex with the addition of guanine due to the strong interaction between guanine and Cu(2+), which caused the fluorescence enhancement. The enhanced fluorescence intensity was well proportional to the concentration of guanine in the range of 4.96 × 10(-8)-1.09 × 10(-6)mol/L with the limit of detection 1.9 × 10(-8)mol/L. The method has been applied successfully to the determination of guanine in serum and DNA samples, and the recoveries were from 96.0% to 104.8%.

Keywords
Cu(2+); Fluorescence; Guanine; Nuclear fast red.
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