Ultrasound-Assisted Aqueous Two-Phase System for Extraction and Enrichment of Zanthoxylum armatum Lignans

  • Molecules. 2015 Aug 20;20(8):15273-86. doi: 10.3390/molecules200815273.
Tao Guo  1  2 ,  Dan Su  3 ,  Yan Huang  4 ,  Ya Wang  5 ,  Yong-Hui Li  6
Affiliations
  • 1. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. [email protected].
  • 2. Hainan Provincial Key Laboratory of R & D of Tropical Plants, Hainan Medical University, Haikou 571101, China. [email protected].
  • 3. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. [email protected].
  • 4. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. [email protected].
  • 5. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China. [email protected].
  • 6. Hainan Provincial Key Laboratory of R & D of Tropical Plants, Hainan Medical University, Haikou 571101, China. [email protected].
PMID: 26307958 DOI: 10.3390/molecules200815273
Abstract

In the study, an aqueous two phase system (ATPS) coupled with ultrasound was employed to extract Lignans from Zanthoxylum armatum. Three standard Lignans, namely (-)-fargesin, sesamin and L-asarinin, were used as marker compounds, and extraction was optimized and projected by response surface methodology (RSM) and artificial neural network (ANN). The optimal condition for ATPS with 20% n-propanol and 24% (NH4)2SO4 coupled with ultrasonic-assisted extraction including a solvent to solid ratio of 15:1, a temperature of 40 °C, and a treatment time of 55 min was obtained. Under the condition, the yield of (-)-fargesin increased 15.12%, and the purities of (-)-fargesin, sesamin and L-asarinin reached 2.222%, 1.066%, and 1.583%, with an increase of 44.38%, 25.70%, and 26.34% compared to those extracted with 95% ethanol, respectively. Coefficient of the determined (0.9855) and mean squared error (0.0018) of ANN model suggested good fitness and generalization of the ANN. Taken together, the results showed that ultrasonic-assisted ATPS can be a suitable method for extraction and enrichment of Lignans from Z. armatum.

Keywords
Zanthoxylum armatum; aqueous two-phase system; artificial neural network; lignans; response surface methodology; ultrasonic-assisted extraction.
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