neo-Clerodane Diterpenoids from Scutellaria barbata and Their Inhibitory Effects on LPS-Induced Nitric Oxide Production

  • J Nat Prod. 2015 Sep 25;78(9):2292-6. doi: 10.1021/acs.jnatprod.5b00126.
Eung Tae Yeon  1 ,  Jin Woo Lee  1 ,  Chul Lee  1 ,  Qinghao Jin  1 ,  Hari Jang  1 ,  Dongho Lee  2 ,  Jong Seog Ahn  3 ,  Jin Tae Hong  1 ,  Youngsoo Kim  1 ,  Mi Kyeong Lee  1 ,  Bang Yeon Hwang  1
Affiliations
  • 1. College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
  • 2. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 02841, Korea.
  • 3. Korea Research Institute of Bioscience and Biotechnology , Cheongju 28116, Korea.
PMID: 26331882 DOI: 10.1021/acs.jnatprod.5b00126
Abstract

Three new neo-clerodane Diterpenoids (1-3) along with 12 known compounds (4-15) were isolated from a methanol extract of the aerial parts of Scutellaria barbata. The structures of 1-3 were determined by interpretation of their 1D and 2D NMR spectroscopic data as well as HRESIMS values. All isolated compounds were tested for their inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages. Compounds 1-4, 7, and 10-12 were found to inhibit nitric oxide production with IC50 values ranging from 20.2 to 35.6 μM.