Diterpenoids from the Roots of Euphorbia fischeriana with Inhibitory Effects on Nitric Oxide Production

  • J Nat Prod. 2016 Jan 22;79(1):126-31. doi: 10.1021/acs.jnatprod.5b00789.
Jin Woo Lee  1 ,  Chul Lee  1 ,  Qinghao Jin  1 ,  Hari Jang  1 ,  Dongho Lee  2 ,  Ha-Jin Lee  3 ,  Jong Won Shin  4 ,  Sang Bae Han  1 ,  Jin Tae Hong  1 ,  Youngsoo Kim  1 ,  Mi Kyeong Lee  1 ,  Bang Yeon Hwang  1
Affiliations
  • 1. College of Pharmacy, Chungbuk National University , Cheongju 28644, Korea.
  • 2. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University , Seoul 02841, Korea.
  • 3. Western Seoul Center, Korea Basic Science Institute , Seoul 03759, Korea.
  • 4. Beamline Department, Pohang Accelerator Laboratory/POSTECH , Pohang 37673, Korea.
PMID: 26702644 DOI: 10.1021/acs.jnatprod.5b00789
Abstract

Bioactivity-guided isolation of a methanolic extract of Euphorbia fischeriana led to the isolation of four new abietane-type Diterpenoids, fischeriolides A-D (1-4), together with 11 known Diterpenoids. Their structures were elucidated based on the interpretation of 1D and 2D NMR spectroscopic and HRESIMS data. The absolute configuration of compound 3 was determined by single-crystal X-ray diffraction analysis and electronic circular dichroism methods. Compounds 5-9 exhibited inhibitory effects on LPS-induced nitric oxide production in RAW 264.7 macrophages with IC50 values in the range 4.9-12.6 μM.