Rosmarinic Acid Attenuates Cell Damage against UVB Radiation-Induced Oxidative Stress via Enhancing Antioxidant Effects in Human HaCaT Cells

  • Biomol Ther (Seoul). 2016 Jan;24(1):75-84. doi: 10.4062/biomolther.2015.069.
Pattage Madushan Dilhara Jayatissa Fernando  1 ,  Mei Jing Piao  1 ,  Kyoung Ah Kang  1 ,  Yea Seong Ryu  1 ,  Susara Ruwan Kumara Madduma Hewage  1 ,  Sung Wook Chae  2 ,  Jin Won Hyun  1
Affiliations
  • 1. Department of Biochemistry, School of Medicine, Jeju National University, Jeju 63243, Republic of Korea.
  • 2. Aging Research Center, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of Korea.
PMID: 26759705 DOI: 10.4062/biomolther.2015.069
Abstract

This study was designed to investigate the cytoprotective effect of rosmarinic acid (RA) on ultraviolet B (UVB)-induced oxidative stress in HaCaT keratinocytes. RA exerted a significant cytoprotective effect by scavenging intracellular ROS induced by UVB. RA also attenuated UVB-induced oxidative macromolecular damage, including protein carbonyl content, DNA strand breaks, and the level of 8-isoprostane. Furthermore, RA increased the expression and activity of superoxide dismutase, catalase, heme oxygenase-1, and their transcription factor Nrf2, which are decreased by UVB radiation. Collectively, these data indicate that RA can provide substantial cytoprotection against the adverse effects of UVB radiation by modulating cellular antioxidant systems, and has potential to be developed as a medical agent for ROS-induced skin diseases.

Keywords
Antioxidant system; Oxidative stress; Reactive oxygen species; Rosmarinic acid; Ultraviolet B.
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