2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition

  • Science. 2016 Feb 19;351(6275):871-5. doi: 10.1126/science.aad7974.
Soojay Banerjee  1 ,  Alberto Bartesaghi  1 ,  Alan Merk  1 ,  Prashant Rao  1 ,  Stacie L Bulfer  2 ,  Yongzhao Yan  3 ,  Neal Green  4 ,  Barbara Mroczkowski  5 ,  R Jeffrey Neitz  2 ,  Peter Wipf  3 ,  Veronica Falconieri  1 ,  Raymond J Deshaies  6 ,  Jacqueline L S Milne  1 ,  Donna Huryn  3 ,  Michelle Arkin  2 ,  Sriram Subramaniam  7
Affiliations
  • 1. Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892, USA.
  • 2. Small Molecule Discovery Center, Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, CA 94143, USA.
  • 3. University of Pittsburgh Chemical Diversity Center, University of Pittsburgh, Pittsburgh, PA 15260, USA.
  • 4. Leidos Biomedical Research Inc., Frederick, MD 21702, USA.
  • 5. Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, MD 20892, USA.
  • 6. Division of Biology and Biological Engineering and Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91107, USA.
  • 7. Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD 20892, USA. [email protected].
PMID: 26822609 DOI: 10.1126/science.aad7974
Abstract

p97 is a hexameric AAA+ adenosine triphosphatase (ATPase) that is an attractive target for Cancer drug development. We report cryo-electron microscopy (cryo-EM) structures for adenosine diphosphate (ADP)-bound, full-length, hexameric wild-type p97 in the presence and absence of an allosteric inhibitor at resolutions of 2.3 and 2.4 angstroms, respectively. We also report cryo-EM structures (at resolutions of ~3.3, 3.2, and 3.3 angstroms, respectively) for three distinct, coexisting functional states of p97 with occupancies of zero, one, or two molecules of adenosine 5'-O-(3-thiotriphosphate) (ATPγS) per protomer. A large corkscrew-like change in molecular architecture, coupled with upward displacement of the N-terminal domain, is observed only when ATPγS is bound to both the D1 and D2 domains of the protomer. These cryo-EM structures establish the sequence of nucleotide-driven structural changes in p97 at atomic resolution. They also enable elucidation of the binding mode of an allosteric small-molecule inhibitor to p97 and illustrate how inhibitor binding at the interface between the D1 and D2 domains prevents propagation of the conformational changes necessary for p97 function.

Products
  • Cat. No.
    Product Name
    Description
    Target
    Research Area
  • HY-123636
    UPCDC-30245
    99.85%, Allosteric p97 Inhibitor
    target: p97
    Research Areas: Cancer