Licoflavonol is an inhibitor of the type three secretion system of Salmonella enterica serovar Typhimurium

  • Biochem Biophys Res Commun. 2016 Sep 2;477(4):998-1004. doi: 10.1016/j.bbrc.2016.07.018.
Zhixing Guo  1 ,  Xiaoli Li  2 ,  Jianfang Li  1 ,  Xuefei Yang  2 ,  Yuan Zhou  1 ,  Chunhua Lu  3 ,  Yuemao Shen  4
Affiliations
  • 1. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, China.
  • 2. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
  • 3. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, China. Electronic address: [email protected].
  • 4. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, China; State Key Laboratory of Microbial Technology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China. Electronic address: [email protected].
PMID: 27387231 DOI: 10.1016/j.bbrc.2016.07.018
Abstract

As an important food-borne human pathogen, Salmonella enterica serovar Typhimurium depends on its type III secretion system (T3SS) as a major virulence factor to cause disease all over the world. The T3SS secretes effector proteins to facilitate invasion into host cells. In this study, twenty prenylated Flavonoids (1-20) were screened for their anti-T3SS activity, revealing that several analogs exhibited strong inhibitory effects on the secretion of Salmonella pathogenicity island 1 (SPI-1)-associated effector proteins without affecting the growth of bacteria and the secretion of the flagellar protein FliC. Among the Flavonoids 1-20, licoflavonol (20) exhibited a strong inhibitory effect on the secretion of the SPI-1 effector proteins via regulating the transcription of the SicA/InvF genes, and the transportation of the effector protein SipC. In summary, licoflavonol, a novel natural inhibitor of Salmonella T3SS, could be a promising candidate for novel type of anti-virulence drugs.

Keywords
Licoflavonol; Salmonella enterica serovar Typhimurium; Type three secretion system (T3SS).
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