Guidelines for the use of flow cytometry and cell sorting in immunological studies

Eur J Immunol. 2017 Oct;47(10):1584-1797. doi: 10.1002/eji.201646632.

Andrea Cossarizza ¹ , Hyun-Dong Chang ² , Andreas Radbruch ² , Mübeccel Akdis ³ , Immanuel Andrä ⁴ , Francesco Annunziato ⁵ , Petra Bacher ⁶ , Vincenzo Barnaba ⁷ ⁸ , Luca Battistini ⁹ , Wolfgang M Bauer ¹⁰ , Sabine Baumgart ² , Burkhard Becher ¹¹ , Wolfgang Beisker ¹² , Claudia Berek ² , Alfonso Blanco ¹³ , Giovanna Borsellino ⁹ , Philip E Boulais ¹⁴ ¹⁵ , Ryan R Brinkman ¹⁶ ¹⁷ , Martin Büscher ¹⁸ , Dirk H Busch ⁴ ¹⁹ ²⁰ , Timothy P Bushnell ²¹ , Xuetao Cao ²² ²³ ²⁴ , Andrea Cavani ²⁵ , Pratip K Chattopadhyay ²⁶ , Qingyu Cheng ²⁷ , Sue Chow ²⁸ , Mario Clerici ²⁹ , Anne Cooke ³⁰ , Antonio Cosma ³¹ , Lorenzo Cosmi ³² , Ana Cumano ³³ , Van Duc Dang ² , Derek Davies ³⁴ , Sara De Biasi ³⁵ , Genny Del Zotto ³⁶ , Silvia Della Bella ³⁷ ³⁸ , Paolo Dellabona ³⁹ , Günnur Deniz ⁴⁰ , Mark Dessing ⁴¹ , Andreas Diefenbach ⁶ , James Di Santo ⁴² , Francesco Dieli ⁴³ , Andreas Dolf ⁴⁴ , Vera S Donnenberg ⁴⁵ , Thomas Dörner ⁴⁶ , Götz R A Ehrhardt ⁴⁷ , Elmar Endl ⁴⁸ , Pablo Engel ⁴⁹ , Britta Engelhardt ⁵⁰ , Charlotte Esser ⁵¹ , Bart Everts ⁵² , Anita Dreher ³ , Christine S Falk ⁵³ ⁵⁴ , Todd A Fehniger ⁵⁵ , Andrew Filby ⁵⁶ , Simon Fillatreau ⁵⁷ ⁵⁸ ⁵⁹ , Marie Follo ⁶⁰ , Irmgard Förster ⁶¹ , John Foster ⁶² , Gemma A Foulds ⁶³ , Paul S Frenette ¹⁴ ⁶⁴ , David Galbraith ⁶⁵ , Natalio Garbi ⁴⁴ ⁶⁶ , Maria Dolores García-Godoy ⁶⁷ , Jens Geginat ⁶⁸ , Kamran Ghoreschi ⁶⁹ , Lara Gibellini ³⁵ , Christoph Goettlinger ⁷⁰ , Carl S Goodyear ⁷¹ , Andrea Gori ⁷² , Jane Grogan ⁷³ , Mor Gross ⁷⁴ , Andreas Grützkau ² , Daryl Grummitt ⁶² , Jonas Hahn ⁷⁵ , Quirin Hammer ² , Anja E Hauser ² ⁷⁶ , David L Haviland ⁷⁷ , David Hedley ²⁸ , Guadalupe Herrera ⁷⁸ , Martin Herrmann ⁷⁵ , Falk Hiepe ²⁷ , Tristan Holland ⁶⁶ , Pleun Hombrink ⁷⁹ , Jessica P Houston ⁸⁰ , Bimba F Hoyer ²⁷ , Bo Huang ⁸¹ ⁸² ⁸³ , Christopher A Hunter ⁸⁴ , Anna Iannone ⁸⁵ , Hans-Martin Jäck ⁸⁶ , Beatriz Jávega ⁸⁷ , Stipan Jonjic ⁸⁸ ⁸⁹ , Kerstin Juelke ² , Steffen Jung ⁷⁴ , Toralf Kaiser ² , Tomas Kalina ⁹⁰ , Baerbel Keller ⁹¹ , Srijit Khan ⁴⁷ , Deborah Kienhöfer ⁷⁵ , Thomas Kroneis ⁹² , Désirée Kunkel ⁹³ , Christian Kurts ⁴⁴ , Pia Kvistborg ⁹⁴ , Joanne Lannigan ⁹⁵ , Olivier Lantz ⁹⁶ ⁹⁷ ⁹⁸ , Anis Larbi ⁹⁹ ¹⁰⁰ ¹⁰¹ ¹⁰² , Salome LeibundGut-Landmann ¹⁰³ , Michael D Leipold ¹⁰⁴ , Megan K Levings ¹⁰⁵ , Virginia Litwin ¹⁰⁶ , Yanling Liu ⁴⁷ , Michael Lohoff ¹⁰⁷ , Giovanna Lombardi ¹⁰⁸ , Lilly Lopez ¹⁰⁹ , Amy Lovett-Racke ¹¹⁰ , Erik Lubberts ¹¹¹ , Burkhard Ludewig ¹¹² , Enrico Lugli ¹¹³ ¹¹⁴ , Holden T Maecker ¹⁰⁴ , Glòria Martrus ¹¹⁵ , Giuseppe Matarese ¹¹⁶ , Christian Maueröder ⁷⁵ , Mairi McGrath ² , Iain McInnes ⁷¹ , Henrik E Mei ² , Fritz Melchers ¹¹⁷ , Susanne Melzer ¹¹⁸ , Dirk Mielenz ¹¹⁹ , Kingston Mills ¹²⁰ , David Mirrer ³ , Jenny Mjösberg ¹²¹ ¹²² , Jonni Moore ¹²³ , Barry Moran ¹²⁰ , Alessandro Moretta ¹²⁴ ¹²⁵ , Lorenzo Moretta ¹²⁶ , Tim R Mosmann ¹²⁷ , Susann Müller ¹²⁸ , Werner Müller ¹²⁹ , Christian Münz ¹¹ , Gabriele Multhoff ¹³⁰ ¹³¹ , Luis Enrique Munoz ⁷⁵ , Kenneth M Murphy ¹³² ¹³³ , Toshinori Nakayama ¹³⁴ , Milena Nasi ³⁵ , Christine Neudörfl ⁵³ , John Nolan ¹³⁵ , Sussan Nourshargh ¹³⁶ , José-Enrique O'Connor ⁸⁷ , Wenjun Ouyang ¹³⁷ , Annette Oxenius ¹³⁸ , Raghav Palankar ¹³⁹ , Isabel Panse ¹⁴⁰ , Pärt Peterson ¹⁴¹ , Christian Peth ¹⁸ , Jordi Petriz ⁶⁷ , Daisy Philips ⁹⁴ , Winfried Pickl ¹⁴² , Silvia Piconese ⁷ ⁸ , Marcello Pinti ¹⁴³ , A Graham Pockley ⁶³ ¹⁴⁴ , Malgorzata Justyna Podolska ⁷⁵ , Carlo Pucillo ¹⁴⁵ , Sally A Quataert ¹²⁷ , Timothy R D J Radstake ¹⁴⁶ , Bartek Rajwa ¹⁴⁷ , Jonathan A Rebhahn ¹²⁷ , Diether Recktenwald ¹⁴⁸ , Ester B M Remmerswaal ¹⁴⁹ , Katy Rezvani ¹⁵⁰ , Laura G Rico ⁶⁷ , J Paul Robinson ¹⁵¹ , Chiara Romagnani ² , Anna Rubartelli ¹⁵² , Beate Ruckert ³ , Jürgen Ruland ¹⁵³ ¹⁵⁴ ¹⁵⁵ , Shimon Sakaguchi ¹⁵⁶ ¹⁵⁷ , Francisco Sala-de-Oyanguren ⁸⁷ , Yvonne Samstag ¹⁵⁸ , Sharon Sanderson ¹⁵⁹ , Birgit Sawitzki ¹⁶⁰ ¹⁶¹ , Alexander Scheffold ² ⁶ , Matthias Schiemann ⁴ , Frank Schildberg ¹⁶² , Esther Schimisky ¹⁶³ , Stephan A Schmid ¹⁶⁴ , Steffen Schmitt ¹⁶⁵ , Kilian Schober ⁴ , Thomas Schüler ¹⁶⁶ , Axel Ronald Schulz ² , Ton Schumacher ⁹⁴ , Cristiano Scotta ¹⁰⁸ , T Vincent Shankey ¹⁶⁷ , Anat Shemer ⁷⁴ , Anna-Katharina Simon ¹⁴⁰ , Josef Spidlen ¹⁶ , Alan M Stall ¹⁶⁸ , Regina Stark ⁷⁹ , Christina Stehle ² , Merle Stein ¹¹⁹ , Tobit Steinmetz ¹¹⁹ , Hannes Stockinger ¹⁶⁹ , Yousuke Takahama ¹⁷⁰ , Attila Tarnok ¹⁷¹ ¹⁷² , ZhiGang Tian ¹⁷³ ¹⁷⁴ , Gergely Toldi ¹⁷⁵ , Julia Tornack ¹¹⁷ , Elisabetta Traggiai ¹⁷⁶ , Joe Trotter ¹⁶⁸ , Henning Ulrich ¹⁷⁷ , Marlous van der Braber ⁹⁴ , René A W van Lier ⁷⁹ , Marc Veldhoen ¹⁷⁸ , Salvador Vento-Asturias ⁶⁶ , Paulo Vieira ¹⁷⁹ , David Voehringer ¹⁸⁰ , Hans-Dieter Volk ¹⁸¹ , Konrad von Volkmann ¹⁸² , Ari Waisman ¹⁸³ , Rachael Walker ¹⁸⁴ , Michael D Ward ¹⁸⁵ , Klaus Warnatz ⁹¹ , Sarah Warth ⁹³ , James V Watson ¹⁸⁶ , Carsten Watzl ¹⁸⁷ , Leonie Wegener ¹⁸ , Annika Wiedemann ⁴⁶ , Jürgen Wienands ¹⁸⁸ , Gerald Willimsky ¹⁸⁹ , James Wing ¹⁵⁶ ¹⁵⁷ , Peter Wurst ⁴⁴ , Liping Yu ¹⁹⁰ , Alice Yue ¹⁹¹ , Qianjun Zhang ¹⁹² , Yi Zhao ¹⁹³ , Susanne Ziegler ¹¹⁵ , Jakob Zimmermann ¹⁹⁴

Affiliations

1. Department of Medical and Surgical Sciences for Children and Adults, Univ. of Modena and Reggio Emilia School of Medicine, Modena, Italy.
2. Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
3. Swiss Institute of Allergy and Asthma Research (SIAF), University Zurich, Davos, Switzerland.
4. Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
5. University of Florence, Internal Medicine, Florence, Italy.
6. Charité - Universitätsmedizin Berlin, Germany.
7. Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Via Regina Elena 324, 00161 Rome, Italy.
8. Istituto Pasteur Italia-Fondazione Cenci Bolognetti, Rome, Italy.
9. Neuroimmunology and Flow Cytometry Units, Santa Lucia Foundation, Rome, Italy.
10. Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
11. University of Zurich, Institute of Experimental Immunology, Zürich, Switzerland.
12. Flow Cytometry Laboratory, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München, German Research Center for Environmental Health.
13. Flow Cytometry Core Technologies, UCD Conway Institute, University College Dublin, Dublin, Ireland.
14. Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
15. The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Bronx, New York, USA.
16. Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC, Canada.
17. Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada.
18. Biopyhsics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
19. DZIF - National Centre for Infection Research, Munich, Germany.
20. Focus Group ''Clinical Cell Processing and Purification", Institute for Advanced Study, Technische Universität München, Munich, Germany.
21. Department of Pediatrics and Shared Resource Laboratories, University of Rochester Medical Center, Rochester NY, United States of America.
22. Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
23. National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai 200433, China.
24. Department of Immunology & Center for Immunotherapy, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China.
25. INMP/NIHMP, Rome, Italy.
26. Immunotechnology Section, Vaccine Research Center, NIH, Bethesda, MD, USA.
27. Medizinische Klinik mit Schwerpunkt Rheumatologie und Medizinische Immunolologie Charité Universitätsmedizin Berlin, Berlin, Germany.
28. Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, Ontario, Canada.
29. University of Milano and Don C Gnocchi Foundation IRCCS, Milano, Italy.
30. Department of Pathology, University of Cambridge, Cambridge, United Kingdom.
31. CEA - Université Paris Sud - INSERM U, Immunology of viral infections and autoimmune diseases, France.
32. Department of Experimental and Clinical Medicine, University of Firenze, Firenze, Italia.
33. Lymphopoiesis Unit, Immunology Department Pasteur Institute, Paris, France.
34. Flow Cytometry Facility, The Francis Crick Institute, London, United Kingdom.
35. Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
36. Istituto Giannina Gaslini, Genova, Italy.
37. University of Milan, Department of Medical Biotechnologies and Translational Medicine.
38. Humanitas Clinical and Research Center, Lab of Clinical and Experimental Immunology, Rozzano, Milan, Italy.
39. Experimental Immunology Unit, Head, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milano, Italy.
40. Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey.
41. Sony Europe Ltd, Weybridge, UK.
42. Institut Pasteur, Immunology, Paris, France.
43. University of Palermo, Department of Biopathology, Palermo, Italy.
44. Institute of Experimental Immunology, University Bonn, Bonn, Germany.
45. Department of Cardiothoracic Surgery, School of Medicine, University of Pittsburgh, PA.
46. Department of Medicine/Rheumatology and Clinical Immunology, Charite Universitätsmedizin Berlin, Germany.
47. Department of Immunology, University of Toronto, Toronto, Canada.
48. Department of Molecular Medicine and Experimental Immunology, (Core Facility Flow Cytometry) University of Bonn, Germany.
49. Department of Biomedical Sciences, University of Barcelona, Barcelona, Spain.
50. Professor for Immunobiology, Director, Theodor Kocher Institute, University of Bern, Bern, Switzerland.
51. IUF - Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany.
52. Leiden University Medical Center, Department of Parasitology, Leiden, The Netherlands.
53. Institute of Transplant Immunology, IFB-Tx, MHH Hannover Medical School, Hannover, Germany.
54. German Center for Infectious diseases (DZIF), TTU-IICH, Hannover, Germany.
55. Divisions of Hematology & Oncology, Department of Medicine, Washington University School of Medicine, St Louis, MO.
56. The Flow Cytometry Core Facility, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.
57. Institut Necker-Enfants Malades (INEM), INSERM U-CNRS UMR, Paris, France.
58. Université Paris Descartes, Sorbonne Paris Cité, Faculté de Médecine, Paris, France.
59. Assistance Publique - Hôpitaux de Paris (AP-HP), Hôpital Necker Enfants Malades, Paris, France.
60. Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
61. Immunology and Environment, Life & Medical Sciences (LIMES) Institute, University of Bonn, Bonn, Germany.
62. Owl Biomedical Inc., Santa Barbara, USA.
63. John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK.
64. Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA.
65. University of Arizona, Bio Institute, School of Plant Sciences and Arizona Cancer Center, Tucson, Arizona, USA.
66. Department of Molecular Immunology, Institute of Experimental Immunology, Bonn, Germany.
67. Josep Carreras Leukemia Research Institute, Barcelona, Spain.
68. INGM, Istituto Nazionale Genetica Molecolare "Romeo ed Enrica Invernizzi", Milan, Italy.
69. Flow Cytometry Core Facility, Department of Dermatology, University Medical Center, Eberhard Karls University Tübingen, Germany.
70. Institut fuer Genetik, Universitaet zu Koeln, Koeln, Germany.
71. Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow.
72. Clinic of Infectious Diseases, "San Gerardo" Hospital - ASST Monza, University Milano-Bicocca, Monza, Italy.
73. Genentech, Department of Cancer Immunology, South San Francisco, California, USA.
74. Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.
75. Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Internal Medicine, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.
76. Immundynamics, Charité - Universitätsmedizin Berlin, Berlin, Germany.
77. Houston Methodist Hospital Research Institute, Houston, TX, USA.
78. Cytometry Service, Incliva Foundation. Clinic Hospital and Faculty of Medicine, The University of Valencia. Av. Blasco Ibáñez, Valencia, Spain.
79. Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam, The Netherlands.
80. Chemical and Materials Engineering, New Mexico State University, Las Cruces, NM, 88003, USA.
81. Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
82. Department of Immunology, Institute of Basic Medical Sciences & State Key Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
83. Clinical Immunology Center, Chinese Academy of Medical Sciences, Beijing, China.
84. Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
85. Department of Diagnostic Medicine, Clinical and Public Health, Univ. of Modena and Reggio Emilia, Modena, Italy.
86. Division of Molecular Immunology, Internal Medicine III, Nikolaus-Fiebiger-Center of MolecularMedicine, University Hospital Erlangen, Erlangen, Germany.
87. Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, The University of Valencia. Av. Blasco Ibáñez, Valencia, Spain.
88. Faculty of Medicine, Center for Proteomics, University of Rijeka, Rijeka, Croatia.
89. Department for Histology and Embryology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
90. Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic.
91. Center for Chronic Immunodeficiency (CCI), Faculty of Medicine, University of Freiburg, Freiburg, Germany.
92. Medical University of Graz, Institute of Cell Biology, Histology & Embryology, Graz, Austria.
93. BCRT Flow Cytometry Lab, Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin.
94. Division of immunology, the Netherlands Cancer Institute, Amsterdam.
95. University of Virginia School of Medicine, Flow Cytometry Shared Resource, Charlottesville, VA, USA.
96. INSERM U932, Institut Curie, Paris 75005, France.
97. Laboratoire d'immunologie clinique, Institut Curie, Paris 75005, France.
98. Centre d'investigation Clinique en Biothérapie Gustave-Roussy Institut Curie (CIC-BT1428), Institut Curie, Paris 75005, France.
99. Singapore Immunology Network (SIgN), Principal Investigator, Biology of Aging Program.
100. Director Flow Cytomerty Platform, Immunomonitoring Platform, Agency for Science Technology and Research (A*STAR), Singapore.
101. Department of Medicine, University of Sherbrooke, Qc, Canada.
102. Faculty of Sciences, ElManar University, Tunis, Tunisia.
103. Section of Immunology, Vetsuisse Faculty, University of Zurich, Switzerland.
104. The Human Immune Monitoring Center (HIMC), Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, CA, USA.
105. Department of Surgery, University of British Columbia & British Columbia Children's Hospital Research Institute, Vancouver, BC, Canada.
106. Covance, Hematology, Indianapolis, IN, USA.
107. Institute for Medical Microbiology and Hospital Hygiene, University of Marburg, Marburg 35043, Germany.
108. MRC Centre for Transplantation, King's College London, Guy's Hospital, SE1 9RT London, UK.
109. Beckman Coulter, Inc, Miami, FL, USA.
110. Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH, USA.
111. Erasmus MC, University Medical Center, Department of Rheumatology, Rotterdam, The Netherlands.
112. Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland.
113. Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy.
114. Humanitas Flow Cytometry Core, Humanitas Clinical and Research Center, Rozzano, Milan, Italy.
115. Department of Virus Immunology, Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
116. Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Napoli, Italy and Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), Napoli, Italy.
117. Senior Group on Lymphocyte Development, Max Planck Institute for Infection Biology, Berlin, Germany.
118. Clinical Trial Center Leipzig, University Leipzig, Leipzig, Germany.
119. Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
120. Trinity Biomedical Sciences Institute, Trinity College Dublin, the University of Dublin, Dublin, Ireland.
121. Center for Infectious Medicine, Department of Medicine, Karolinska Institute Stockholm, Sweden.
122. Department of Clinical and Experimental Medicine, Linköping University, Sweden.
123. Department of Pathology and Laboratory Medicine, Perelman School of Medicine of the University of Pennsylvania, Philadelphia, Pennsylvania.
124. Department of Experimental Medicine, University of Genova, Genova, Italy.
125. Centro di Eccellenza per la Ricerca Biomedica-CEBR, Genova, Italy.
126. Department of Immunology, IRCCS Bambino Gesu Children's Hospital, Rome, Italy.
127. David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
128. Centre for Environmental Research - UFZ, Department Environemntal Microbiology, Leipzig, Germany.
129. Bill Ford Chair in Cellular Immunology, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom.
130. Department of Radiation Oncology, Klinikum rechts der Isar, Technische Universität München (TUM), Munich, Germany.
131. Institute for Innovative Radiotherapy (iRT), Experimental Immune Biology, Helmholtz Zentrum München, Neuherberg, Germany.
132. Department of Pathology and Immunology, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
133. Howard Hughes Medical Institute, School of Medicine, Washington University in St. Louis, St. Louis, MO, USA.
134. Department of Immunology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan.
135. The Scintillon Institute, Nancy Ridge Drive, San Diego, CA, USA.
136. Centre for Microvascular Research, William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom.
137. Department of Inflammation and Oncology, Amgen Inc., South San Francisco, CA, USA.
138. Institute of Microbiology, ETH Zurich, Zurich, Switzerland.
139. Institute for Immunology and Transfusion Medicine, University Medicine Greifswald, Ferdinand-Sauerbruch-Straße, 17489, Greifswald, Germany.
140. Kennedy Institute of Rheumatology, University of Oxford, Oxford, United Kingdom.
141. Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
142. Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
143. Department of Life Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
144. Chromocyte Limited, Electric Works, Sheffield, UK.
145. Univeristy of Udine - Department of Medicine, Lab of Immunology, Udine, Italy.
146. Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht, The Netherlands; Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht, The Netherlands.
147. Bindley Biosciences Center, Purdue University, West Lafayette, In, USA.
148. Desatoya LLC, Reno, NV, USA.
149. Department of Experimental Immunology and Renal Transplant Unit, Division of Internal Medicine, Academic Medical Centre, The Netherlands.
150. Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
151. The SVM Professor of Cytomics & Professor of Biomedical Engineering, Purdue University Cytometry Laboratories, Purdue University, West Lafayette, IN, USA.
152. Cell Biology Unit, IRCCS AOU San Martino-IST, Genova, Italy.
153. Institut für Klinische Chemie und Pathobiochemie, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
154. German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.
155. German Center for Infection Research (DZIF), partner site Munich, Munich, Germany.
156. Laboratory of Experimental Immunology, WPI Immunology Frontier Research Center (IFReC), Osaka University, Suita 565-0871, Japan.
157. Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
158. Institute of Immunology, Section Molecular Immunology, Ruprecht-Karls-University, D-69120, Heidelberg, Germany.
159. Translational Immunology Laboratory, NIHR BRC, University of Oxford, Kennedy Institute of Rheumatology,Oxford, United Kingdom.
160. Charité-Universitaetsmedizin Berlin, Corporate Member of Freie Universitaet Berlin, Humboldt-Universitaet zu Berlin.
161. Berlin Institute of Health, Institute of Medical Immunology, Augustenburger Platz 1, 13353 Berlin, Germany.
162. Harvard Medical School, Department of Microbiology and Immunobiology, Boston, MA, USA.
163. R&D Reagents, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
164. Klinik und Poliklinik für Innere Medizin I, Universitätsklinikum Regensburg, Regensburg, Germany.
165. Imaging and Cytometry Core Facility, Flow Cytometry Unit, German Cancer Research Centre (DKFZ), Heidelberg, Germany.
166. Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, Germany.
167. AsedaSciences, West Lafayette, IN, USA.
168. BD Life Sciences, San Diego, CA USA.
169. Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
170. Division of Experimental Immunology, Institute of Advanced Medical Sciences, University of Tokushima, Tokushima, Japan.
171. Departement for Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.
172. Institute for Medical Informatics, IMISE, Leipzig, Germany.
173. School of Life Sciences and Medical Center, Institute of Immunology, Key Laboratory of Innate Immunity and Chronic Disease of Chinese Academy of Science, University of Science and Technology of China, Hefei, China.
174. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
175. University of Birmingham, Institute of Immunology and Immunotherapy, Birmingham, UK.
176. Novartis Institute for Biomedical Research (NIBR), Basel Switzerland.
177. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo.
178. Instituto de Medicina Molecular, Lisbon, Portugal.
179. Unité Lymphopoiese, Institut Pasteur, Paris, France.
180. Department of Infection Biology, University Hospital Erlangen, Wasserturmstr. 3/5, 91054 Erlangen, Germany.
181. Inst. Med. Immunol. & BCRT & Labor Berlin.
182. A·P·E-Applied Physics & Electronics, Berlin, Germany.
183. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany.
184. Babraham Institute, Cambridge, UK.
185. Thermo Fisher Scientific, Eugene, Oregon, USA.
186. Medinfomatics Ltd., West Drayton, Middlesex, England.
187. Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund, IfADo, Department of Immunology, Dortmund, Germany.
188. Universitätsmedizin Göttingen, Georg-August-Universität, Abt. Zelluläre und Molekulare Immunologie, Humboldtallee 34, 37073 Göttingen, Germany.
189. Cooperation Unit for Experimental and Translational Cancer Immunology, Institute of Immunology (Charité - Universitätsmedizin Berlin) and German Cancer Research Center (DKFZ), Berlin, Germany.
190. BD Biosciences, San Jose CA, USA.
191. School of Computing Science, Simon Fraser University, Burnaby, Canada.
192. Emerald Biotech Co., Ltd, Hangzhou, China.
193. Department of Rheumatology & Immunology, West China Hospital, Sichuan University, Chengdu, China.
194. Maurice Müller Laboratories (DKF), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Murtenstrasse, Bern.

PMID: 29023707 DOI: 10.1002/eji.201646632