FACS single cell index sorting is highly reliable and determines immune phenotypes of clonally expanded T cells

  • Eur J Immunol. 2018 Jul;48(7):1248-1250. doi: 10.1002/eji.201847507.
Livius Penter  1  2 ,  Kerstin Dietze  1 ,  Lars Bullinger  1 ,  Jörg Westermann  1 ,  Hans-Peter Rahn  3 ,  Leo Hansmann  1  2
Affiliations
  • 1. Department of Hematology, Oncology, and Tumor Immunology, Charité - Universitätsmedizin Berlin, Berlin, Germany.
  • 2. Berlin Institute of Health (BIH), Berlin, Germany.
  • 3. Flow Cytometry Technology Platform, Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany.
PMID: 29537492 DOI: 10.1002/eji.201847507
Abstract

FACS index sorting allows the isolation of single cells with retrospective identification of each single cell's high-dimensional immune phenotype. We experimentally determine the error rate of index sorting and combine the technology with T cell receptor Sequencing to identify clonal T cell expansion in aplastic anemia bone marrow as an example.

Keywords
Aplastic anemia; Index sorting; Multicolor flow cytometry; Single cell technologies; T cells.