Gö6983 attenuates titanium particle-induced osteolysis and RANKL mediated osteoclastogenesis through the suppression of NFκB/JNK/p38 pathways
- Biochem Biophys Res Commun. 2018 Sep 3;503(1):62-70. doi: 10.1016/j.bbrc.2018.05.177.
- 1. Departments of Orthopedics, The First Affliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi, China.
- 2. Departments of Pathology, The First Affliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
- 3. Departments of General Medicine, The First Affliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
- 4. Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi, China.
- 5. Departments of Cardiothoracic Surgery, The First Affliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
- 6. Departments of Orthopedics, The First Affliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi, China. Electronic address: [email protected].
- 7. Departments of Orthopedics, The First Affliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi, China. Electronic address: [email protected].
- 8. Departments of Orthopedics, The First Affliated Hospital of Guangxi Medical University, Nanning, Guangxi, China; Research Centre for Regenerative Medicine, Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, Guangxi, China. Electronic address: [email protected].
Osteoclast activation by wear particles has caused major difficulties for surgeons. Wear particles are the main causes of aseptic prosthetic loosening. Gö6983, a protein kinase C inhibitor, inhibits five subtypes of protein kinase C family members. Here, we found that Gö6983 had an obviously inhibitory effect on wear-particles-induced osteolysis in vivo. In vitro, Gö6983 inhibited RANKL-stimulated osteoclast formation and function by inhibiting the RANKL-stimulated nuclear factor-κB/JNK/p38 signaling pathway. We also observed that Go6983 had no effect on the differentiation of osteoblasts and osteoblast-associated genes expression. According to our data, Gö6983 has potential therapeutic effects for aseptic prosthetic loosening caused by osteoclast activation.