In Vitro Study of Antitumor Effect of Antimicrobial Peptide Tachyplesin I in Combination with Cisplatin

  • Bull Exp Biol Med. 2018 Jun;165(2):220-224. doi: 10.1007/s10517-018-4134-6.
D V Kuzmin  1 ,  A A Emel'yanova  1 ,  M B Kalashnikova  1 ,  P V Panteleev  1 ,  T V Ovchinnikova  2
Affiliations
  • 1. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
  • 2. M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. [email protected].
PMID: 29922995 DOI: 10.1007/s10517-018-4134-6
Abstract

We studied combined effect of β-hairpin antimicrobial peptide tachyplesin I and cytotoxic agent cisplatin on tumor and normal human cell lines. MTT assay and flow cytometry showed that tachyplesin I selectively sensitized Cancer cells to cisplatin in specified concentration ratios. In vitro experiments demonstrated that combined use of tachyplesin I and cisplatin allows decreasing the effective dose of the cytostatic thus reducing nonspecific toxicity.

Keywords
antimicrobial peptide; cisplatin; cytotoxicity; effective dose; tachyplesin.
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