Flavonoid profile and radical-scavenging activity of Mediterranean sweet lemon (Citrus limetta Risso) juice

  • Food Chem. 2011 Nov 15;129(2):417-422. doi: 10.1016/j.foodchem.2011.04.093.
Davide Barreca  1 Ersilia Bellocco  1 Corrado Caristi  1 Ugo Leuzzi  1 Giuseppe Gattuso  2
Affiliations
  • 1. Dipartimento di Chimica Organica e Biologica, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy.
  • 2. Dipartimento di Chimica Organica e Biologica, Università di Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy. Electronic address: [email protected].
Abstract

The flavonoid composition of crude Citrus limetta Risso (limetta, Mediterranean sweet lemon) juice was elucidated by means of reverse-phase HPLC-DAD-ESI-MS/MS analysis. In a single course, eight compounds (C- and O-glycosyl Flavonoids) were identified, six of which were found for the first time in limetta juice: four C-glucosyl Flavones, namely vicenin-2, lucenin-2 4'-methyl ether, orientin 4'-methyl ether and scoparin; the O-glycosyl flavone, rhoifolin; and the O-glycosyl flavanone, eriocitrin. In addition, ABTS+ and DPPH radical quenching and FRAP assays demonstrate that limetta juice possesses remarkable antioxidant activity.

Keywords
Antioxidant activity; Citrus limetta Risso; Flavonoids; HPLC–DAD–ESI-MS/MS; Limetta juice; Mediterranean sweet lemon.
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