Isolation and identification of littorine from hairy roots of Atropa belladonna

  • Plant Cell Rep. 1998 Dec;18(3-4):249-251. doi: 10.1007/s002990050565.
F Nakanishi  1 ,  K Sasaki  2 ,  K Shimomura  3
Affiliations
  • 1. Department of Biology, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan Fax: +81-423-29 7737 e-mail: [email protected], , , , , , JP.
  • 2. Department of Bioscience and Biotechnology, Aomori University, Kohbata, Aomori 030-0943, Japan, , , , , , JP.
  • 3. Tsukuba Medicinal Plant Research Station, National Institute of Health Sciences, 1 Hachimanndai Tsukuba, Ibaraki, 305-0843, Japan, , , , , , JP.
PMID: 30744229 DOI: 10.1007/s002990050565
Abstract

A hairy root clone (M8) of Atropa belladonna, producing high levels of Tropane Alkaloids, was established by transformation with Agrobacterium rhizogenes (MAFF 03-01724). Littorine, an intermediate of Tropane Alkaloids, was detected by high-performance liquid chromatography and gas chromatography-mass spectrometry in the alkaloid fraction of the hairy roots and identified by nuclear magnetic resonance analysis. Littorine was also detected in the non-transformed root culture of A. belladonna.

Keywords
Hairy root; Key wordsAtropa belladonna; Littorine; Root culture; Tropane alkaloid.
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