Unmodified household coffee maker assisted extraction and purification of anticancer agents from Dillenia indica fruits

  • Nat Prod Res. 2021 Mar;35(6):984-987. doi: 10.1080/14786419.2019.1608546.
Mohsin Ali Khan  1 ,  Tanveer Ahamad  2 ,  Mohammad Saquib  3 ,  Mohd Kamil Hussain  4 ,  Mohammad Faheem Khan  2  5
Affiliations
  • 1. Research Unit, Era's Lucknow Medical College & Hospital, Era University, Lucknow, UP, India.
  • 2. Department of Biotechnology, Era's Lucknow Medical College & Hospital, Era University, Lucknow, UP, India.
  • 3. Department of Chemistry, University of Allahabad, Allahabad, UP, India.
  • 4. Department of Chemistry, Government Raza Post Graduate College, Rampur, UP, India.
  • 5. Department of Chemistry, Era University, Lucknow, UP, India.
PMID: 31134812 DOI: 10.1080/14786419.2019.1608546
Abstract

Bioassay targeted, 80% aqueous ethanol crude extract of the fruits of Dillenia indica Linn, using the unmodified household coffee maker, afforded five compounds, namely betulinic acid (1), rhamnazin (2), dillenetin (3), luteolin-7-O-β-D-glucoside (4) and hypolaetin-8-O-β-D-glucoside (5). The crude extract, fractions and purified compounds were tested against MDA MB-231, A549 and HeLa Cancer cell lines by MTT assay, using betulinic acid 1, as a positive control. Compound 3 showed the best activity against A549 (IC50 = 26.60 ± 2.5 µM) and HeLa Cancer cell lines (IC50 =19.35 ± 0.9 µM), whereas compound 5 was found to show the best activity against MDA MB-231 (IC50 = 34.62 ± 5.2µM) Cancer cell line. These highly potent Anticancer compounds obtained from the fruits of D. indica may be suitable for herbal drug development and formulations.

Keywords
Dillenia indica; anticancer agents; betulinic acid; household coffee maker.
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