Super-Resolution Microscopy of Chromatin

  • Genes (Basel). 2019 Jun 28;10(7):493. doi: 10.3390/genes10070493.
Udo J Birk  1  2
Affiliations
  • 1. University of Applied Sciences HTW Chur, Pulvermühlestrasse 57, 7004 Chur, Switzerland. [email protected].
  • 2. Institut für Physik, Universität Mainz, 55122 Mainz, Germany. [email protected].
Abstract

Since the advent of super-resolution microscopy, countless approaches and studies have been published contributing significantly to our understanding of cellular processes. With the aid of chromatin-specific fluorescence labeling techniques, we are gaining increasing insight into gene regulation and chromatin organization. Combined with super-resolution imaging and data analysis, these labeling techniques enable direct assessment not only of chromatin interactions but also of the function of specific chromatin conformational states.

Keywords
DNA; DNA labeling; chromatin; fluorescence; nuclear architecture; single molecule localization microscopy; super-resolution microscopy.