Identification of moracin N in mulberry leaf and evaluation of antioxidant activity

  • Food Chem Toxicol. 2019 Oct:132:110730. doi: 10.1016/j.fct.2019.110730.
Jiaming Tu  1 ,  Dingding Shi  1 ,  Lingrong Wen  1 ,  Yueming Jiang  1 ,  Yupeng Zhao  2 ,  Jiali Yang  1 ,  Huiling Liu  1 ,  Guangrong Liu  3 ,  Bao Yang  4
Affiliations
  • 1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • 2. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
  • 3. Infinitus (China) Company Ltd, Guangzhou, 510665, China.
  • 4. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China; University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address: [email protected].
PMID: 31369850 DOI: 10.1016/j.fct.2019.110730
Abstract

Mulberry leaf is a newly accepted vegetable for daily diet. It tastes good and has multiple health benefits, including antioxidant and anti-inflammatory activities. However, the chemicals responsible for these health benefits remain unveiled. Prenylated phenolics are characteristic bioactive compounds in mulberry leaf, which are recognized as good Antioxidants. In this work, moracin N was purified from mulberry leaf. It showed better antioxidant activities than resveratrol. The EC50 value of cellular antioxidant activity was 24.92 μM, and the IC50 value against DPPH radical was 40.00 μM. The prenyl group rendered the molecule more membrane affinity which improved the bioavailability. The furan ring was critical for the antioxidant behaviour. The cell viability test revealed that moracin N had a good safety. These results pointed out that moracin N contributed to the antioxidant activity of mulberry leaf.

Keywords
Cellular antioxidant activity; Mulberry leaf; NMR; Prenylated phenolics; Resveratrol.
Products