Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

  • Eur J Immunol. 2019 Oct;49(10):1457-1973. doi: 10.1002/eji.201970107.
Andrea Cossarizza  1 ,  Hyun-Dong Chang  2 ,  Andreas Radbruch  2 ,  Andreas Acs  3 ,  Dieter Adam  4 ,  Sabine Adam-Klages  5 ,  William W Agace  6  7 ,  Nima Aghaeepour  8 ,  Mübeccel Akdis  9 ,  Matthieu Allez  10 ,  Larissa Nogueira Almeida  11 ,  Giorgia Alvisi  12 ,  Graham Anderson  13 ,  Immanuel Andrä  14 ,  Francesco Annunziato  15 ,  Achille Anselmo  16 ,  Petra Bacher  4  17 ,  Cosima T Baldari  18 ,  Sudipto Bari  19  20 ,  Vincenzo Barnaba  21  22  23 ,  Joana Barros-Martins  24 ,  Luca Battistini  25 ,  Wolfgang Bauer  26 ,  Sabine Baumgart  2 ,  Nicole Baumgarth  27 ,  Dirk Baumjohann  28 ,  Bianka Baying  29 ,  Mary Bebawy  30 ,  Burkhard Becher  31  32 ,  Wolfgang Beisker  33 ,  Vladimir Benes  29 ,  Rudi Beyaert  34 ,  Alfonso Blanco  35 ,  Dominic A Boardman  36  37 ,  Christian Bogdan  38  39 ,  Jessica G Borger  40 ,  Giovanna Borsellino  41 ,  Philip E Boulais  42  43 ,  Jolene A Bradford  44 ,  Dirk Brenner  45  46  47 ,  Ryan R Brinkman  48  49 ,  Anna E S Brooks  50 ,  Dirk H Busch  14  51  52 ,  Martin Büscher  53 ,  Timothy P Bushnell  54 ,  Federica Calzetti  55 ,  Garth Cameron  56 ,  Ilenia Cammarata  21 ,  Xuetao Cao  57 ,  Susanna L Cardell  58 ,  Stefano Casola  59 ,  Marco A Cassatella  55 ,  Andrea Cavani  60 ,  Antonio Celada  61 ,  Lucienne Chatenoud  62 ,  Pratip K Chattopadhyay  63 ,  Sue Chow  64 ,  Eleni Christakou  65  66 ,  Luka Čičin-Šain  67 ,  Mario Clerici  68  69  70 ,  Federico S Colombo  16 ,  Laura Cook  37  71 ,  Anne Cooke  72 ,  Andrea M Cooper  73 ,  Alexandra J Corbett  56 ,  Antonio Cosma  74 ,  Lorenzo Cosmi  15 ,  Pierre G Coulie  75 ,  Ana Cumano  76 ,  Ljiljana Cvetkovic  77 ,  Van Duc Dang  2 ,  Chantip Dang-Heine  78 ,  Martin S Davey  79  80 ,  Derek Davies  81 ,  Sara De Biasi  82 ,  Genny Del Zotto  83 ,  Gelo Victoriano Dela Cruz  84 ,  Michael Delacher  85  86 ,  Silvia Della Bella  87 ,  Paolo Dellabona  88 ,  Günnur Deniz  89 ,  Mark Dessing  90 ,  James P Di Santo  91  92 ,  Andreas Diefenbach  2  93  94 ,  Francesco Dieli  95 ,  Andreas Dolf  96 ,  Thomas Dörner  2  97 ,  Regine J Dress  98 ,  Diana Dudziak  99 ,  Michael Dustin  100 ,  Charles-Antoine Dutertre  101  98 ,  Friederike Ebner  102 ,  Sidonia B G Eckle  56 ,  Matthias Edinger  85  103 ,  Pascale Eede  104 ,  Götz R A Ehrhardt  105 ,  Marcus Eich  106 ,  Pablo Engel  107 ,  Britta Engelhardt  108 ,  Anna Erdei  109 ,  Charlotte Esser  110 ,  Bart Everts  111 ,  Maximilien Evrard  98 ,  Christine S Falk  112 ,  Todd A Fehniger  113 ,  Mar Felipo-Benavent  114 ,  Helen Ferry  115 ,  Markus Feuerer  85  86 ,  Andrew Filby  116 ,  Kata Filkor  117 ,  Simon Fillatreau  118 ,  Marie Follo  119  120 ,  Irmgard Förster  121 ,  John Foster  122 ,  Gemma A Foulds  123 ,  Britta Frehse  11 ,  Paul S Frenette  42  43  124 ,  Stefan Frischbutter  2  125 ,  Wolfgang Fritzsche  126 ,  David W Galbraith  127  128 ,  Anastasia Gangaev  129 ,  Natalio Garbi  130 ,  Brice Gaudilliere  131 ,  Ricardo T Gazzinelli  132  133 ,  Jens Geginat  134 ,  Wilhelm Gerner  135  136 ,  Nicholas A Gherardin  56 ,  Kamran Ghoreschi  137 ,  Lara Gibellini  82 ,  Florent Ginhoux  98  138  139 ,  Keisuke Goda  140  141  142 ,  Dale I Godfrey  56 ,  Christoph Goettlinger  143 ,  Jose M González-Navajas  144  145 ,  Carl S Goodyear  146 ,  Andrea Gori  147 ,  Jane L Grogan  148 ,  Daryl Grummitt  122 ,  Andreas Grützkau  2 ,  Claudia Haftmann  31 ,  Jonas Hahn  149 ,  Hamida Hammad  150 ,  Günter Hämmerling  151 ,  Leo Hansmann  94  152  153 ,  Goran Hansson  154 ,  Christopher M Harpur  155 ,  Susanne Hartmann  102 ,  Andrea Hauser  103 ,  Anja E Hauser  2  156  157 ,  David L Haviland  158 ,  David Hedley  64 ,  Daniela C Hernández  2  159 ,  Guadalupe Herrera  160 ,  Martin Herrmann  149 ,  Christoph Hess  161  162 ,  Thomas Höfer  163 ,  Petra Hoffmann  85  103 ,  Kristin Hogquist  164 ,  Tristan Holland  130 ,  Thomas Höllt  165  166 ,  Rikard Holmdahl  167 ,  Pleun Hombrink  168  169 ,  Jessica P Houston  170 ,  Bimba F Hoyer  171 ,  Bo Huang  172 ,  Fang-Ping Huang  173 ,  Johanna E Huber  28 ,  Jochen Huehn  174 ,  Michael Hundemer  175 ,  Christopher A Hunter  176 ,  William Y K Hwang  177  20  178 ,  Anna Iannone  179 ,  Florian Ingelfinger  31 ,  Sabine M Ivison  36  37 ,  Hans-Martin Jäck  77 ,  Peter K Jani  2  180 ,  Beatriz Jávega  181 ,  Stipan Jonjic  182 ,  Toralf Kaiser  2 ,  Tomas Kalina  183 ,  Thomas Kamradt  184 ,  Stefan H E Kaufmann  180 ,  Baerbel Keller  185  186 ,  Steven L C Ketelaars  129 ,  Ahad Khalilnezhad  98  187 ,  Srijit Khan  105 ,  Jan Kisielow  188 ,  Paul Klenerman  115 ,  Jasmin Knopf  149 ,  Hui-Fern Koay  56 ,  Katja Kobow  189 ,  Jay K Kolls  190 ,  Wan Ting Kong  98 ,  Manfred Kopf  188 ,  Thomas Korn  191 ,  Katharina Kriegsmann  175 ,  Hendy Kristyanto  192 ,  Thomas Kroneis  193 ,  Andreas Krueger  194 ,  Jenny Kühne  112 ,  Christian Kukat  195 ,  Désirée Kunkel  196  197 ,  Heike Kunze-Schumacher  194 ,  Tomohiro Kurosaki  198 ,  Christian Kurts  130 ,  Pia Kvistborg  129 ,  Immanuel Kwok  98  199 ,  Jonathan Landry  29 ,  Olivier Lantz  200 ,  Paola Lanuti  201 ,  Francesca LaRosa  68  70 ,  Agnès Lehuen  202 ,  Salomé LeibundGut-Landmann  203 ,  Michael D Leipold  204 ,  Leslie Y T Leung  105 ,  Megan K Levings  36  37  205 ,  Andreia C Lino  2  97 ,  Francesco Liotta  15 ,  Virginia Litwin  206 ,  Yanling Liu  105 ,  Hans-Gustaf Ljunggren  207 ,  Michael Lohoff  208 ,  Giovanna Lombardi  209 ,  Lilly Lopez  210 ,  Miguel López-Botet  211 ,  Amy E Lovett-Racke  212 ,  Erik Lubberts  213 ,  Herve Luche  214 ,  Burkhard Ludewig  215 ,  Enrico Lugli  12  16 ,  Sebastian Lunemann  216 ,  Holden T Maecker  217 ,  Laura Maggi  15 ,  Orla Maguire  218 ,  Florian Mair  219 ,  Kerstin H Mair  135  136 ,  Alberto Mantovani  220  221 ,  Rudolf A Manz  11 ,  Aaron J Marshall  222 ,  Alicia Martínez-Romero  223 ,  Glòria Martrus  216 ,  Ivana Marventano  68  70 ,  Wlodzimierz Maslinski  224 ,  Giuseppe Matarese  225 ,  Anna Vittoria Mattioli  82  226 ,  Christian Maueröder  227  228 ,  Alessio Mazzoni  15 ,  James McCluskey  56 ,  Mairi McGrath  2 ,  Helen M McGuire  229 ,  Iain B McInnes  146 ,  Henrik E Mei  2 ,  Fritz Melchers  2  180 ,  Susanne Melzer  230 ,  Dirk Mielenz  77 ,  Stephen D Miller  231 ,  Kingston H G Mills  232 ,  Hans Minderman  218 ,  Jenny Mjösberg  207  233 ,  Jonni Moore  234 ,  Barry Moran  232 ,  Lorenzo Moretta  235 ,  Tim R Mosmann  236 ,  Susann Müller  237 ,  Gabriele Multhoff  238  239 ,  Luis Enrique Muñoz  149 ,  Christian Münz  31  32 ,  Toshinori Nakayama  240 ,  Milena Nasi  82 ,  Katrin Neumann  241 ,  Lai Guan Ng  98  187  199  242  243 ,  Antonia Niedobitek  2 ,  Sussan Nourshargh  244 ,  Gabriel Núñez  245 ,  José-Enrique O'Connor  181 ,  Aaron Ochel  241 ,  Anna Oja  169 ,  Diana Ordonez  246 ,  Alberto Orfao  247 ,  Eva Orlowski-Oliver  248 ,  Wenjun Ouyang  249 ,  Annette Oxenius  250 ,  Raghavendra Palankar  251 ,  Isabel Panse  2 ,  Kovit Pattanapanyasat  252 ,  Malte Paulsen  246 ,  Dinko Pavlinic  29 ,  Livius Penter  153 ,  Pärt Peterson  253 ,  Christian Peth  53 ,  Jordi Petriz  254 ,  Federica Piancone  68  70 ,  Winfried F Pickl  255 ,  Silvia Piconese  21  23 ,  Marcello Pinti  256 ,  A Graham Pockley  123  257 ,  Malgorzata Justyna Podolska  149  258 ,  Zhiyong Poon  177 ,  Katharina Pracht  77 ,  Immo Prinz  24 ,  Carlo E M Pucillo  259 ,  Sally A Quataert  236 ,  Linda Quatrini  235 ,  Kylie M Quinn  260  261 ,  Helena Radbruch  104 ,  Tim R D J Radstake  262 ,  Susann Rahmig  263 ,  Hans-Peter Rahn  264 ,  Bartek Rajwa  265 ,  Gevitha Ravichandran  241 ,  Yotam Raz  266 ,  Jonathan A Rebhahn  236 ,  Diether Recktenwald  267 ,  Dorothea Reimer  77 ,  Caetano Reis e Sousa  268 ,  Ester B M Remmerswaal  168  269 ,  Lisa Richter  270 ,  Laura G Rico  254 ,  Andy Riddell  81 ,  Aja M Rieger  271 ,  J Paul Robinson  272 ,  Chiara Romagnani  2  159 ,  Anna Rubartelli  273 ,  Jürgen Ruland  274 ,  Armin Saalmüller  135 ,  Yvan Saeys  275  276 ,  Takashi Saito  277 ,  Shimon Sakaguchi  198 ,  Francisco Sala-de-Oyanguren  278 ,  Yvonne Samstag  279 ,  Sharon Sanderson  280 ,  Inga Sandrock  24 ,  Angela Santoni  281 ,  Ramon Bellmàs Sanz  112 ,  Marina Saresella  68  70 ,  Catherine Sautes-Fridman  282 ,  Birgit Sawitzki  283 ,  Linda Schadt  31  32 ,  Alexander Scheffold  4 ,  Hans U Scherer  192 ,  Matthias Schiemann  14 ,  Frank A Schildberg  284 ,  Esther Schimisky  285 ,  Andreas Schlitzer  286 ,  Josephine Schlosser  102 ,  Stephan Schmid  287 ,  Steffen Schmitt  288 ,  Kilian Schober  14 ,  Daniel Schraivogel  289 ,  Wolfgang Schuh  77 ,  Thomas Schüler  290 ,  Reiner Schulte  291 ,  Axel Ronald Schulz  2 ,  Sebastian R Schulz  77 ,  Cristiano Scottá  209 ,  Daniel Scott-Algara  292 ,  David P Sester  293 ,  T Vincent Shankey  294 ,  Bruno Silva-Santos  295 ,  Anna Katharina Simon  100 ,  Katarzyna M Sitnik  67 ,  Silvano Sozzani  296 ,  Daniel E Speiser  297 ,  Josef Spidlen  298 ,  Anders Stahlberg  299 ,  Alan M Stall  300 ,  Natalie Stanley  8 ,  Regina Stark  168  169 ,  Christina Stehle  2  159 ,  Tobit Steinmetz  77 ,  Hannes Stockinger  301 ,  Yousuke Takahama  302 ,  Kiyoshi Takeda  198 ,  Leonard Tan  98  187 ,  Attila Tárnok  303  304  305 ,  Gisa Tiegs  241 ,  Gergely Toldi  117 ,  Julia Tornack  2  306 ,  Elisabetta Traggiai  307 ,  Mohamed Trebak  308 ,  Timothy I M Tree  65  66 ,  Joe Trotter  300 ,  John Trowsdale  72 ,  Maria Tsoumakidou  309 ,  Henning Ulrich  310 ,  Sophia Urbanczyk  77 ,  Willem van de Veen  9  311 ,  Maries van den Broek  31  32 ,  Edwin van der Pol  312 ,  Sofie Van Gassen  275  276 ,  Gert Van Isterdael  313 ,  René A W van Lier  169 ,  Marc Veldhoen  295 ,  Salvador Vento-Asturias  130 ,  Paulo Vieira  76 ,  David Voehringer  314 ,  Hans-Dieter Volk  315 ,  Anouk von Borstel  79  80 ,  Konrad von Volkmann  316 ,  Ari Waisman  317 ,  Rachael V Walker  318 ,  Paul K Wallace  319 ,  Sa A Wang  320 ,  Xin M Wang  321 ,  Michael D Ward  44 ,  Kirsten A Ward-Hartstonge  36 ,  Klaus Warnatz  185  186 ,  Gary Warnes  322 ,  Sarah Warth  197 ,  Claudia Waskow  263  323 ,  James V Watson  324 ,  Carsten Watzl  325 ,  Leonie Wegener  53 ,  Thomas Weisenburger  3 ,  Annika Wiedemann  2  97 ,  Jürgen Wienands  326 ,  Anneke Wilharm  24 ,  Robert John Wilkinson  327  328  329 ,  Gerald Willimsky  330 ,  James B Wing  198 ,  Rieke Winkelmann  4 ,  Thomas H Winkler  3 ,  Oliver F Wirz  9 ,  Alicia Wong  98 ,  Peter Wurst  331 ,  Jennie H M Yang  65  66 ,  Juhao Yang  174 ,  Maria Yazdanbakhsh  111 ,  Liping Yu  332 ,  Alice Yue  333 ,  Hanlin Zhang  100 ,  Yi Zhao  334 ,  Susanne Maria Ziegler  216 ,  Christina Zielinski  51  335  336 ,  Jakob Zimmermann  337 ,  Arturo Zychlinsky  180
Affiliations
  • 1. Department of Medical and Surgical Sciences for Children and Adults, Univ. of Modena and Reggio Emilia School of Medicine, Modena, Italy.
  • 2. Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
  • 3. Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
  • 4. Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
  • 5. Institut für Transfusionsmedizin, Universitätsklinik Schleswig-Holstein, Kiel, Germany.
  • 6. Mucosal Immunology group, Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.
  • 7. Immunology Section, Lund University, Lund, Sweden.
  • 8. Departments of Anesthesiology, Pain and Perioperative Medicine, Biomedical Data Sciences; and Pediatrics, Stanford University, Stanford, CA, USA.
  • 9. Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland.
  • 10. Université de Paris, Institut de Recherche Saint-Louis, INSERM U1160, and Gastroenterology Department, Hôpital Saint-Louis - APHP, Paris, France.
  • 11. Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany.
  • 12. Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Italy.
  • 13. University of Birmingham, Birmingham, UK.
  • 14. Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
  • 15. Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
  • 16. Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy.
  • 17. Institut für Klinische Molekularbiologie, Christian-Albrechts Universität zu Kiel, Germany.
  • 18. Department of Life Sciences, University of Siena, Siena, Italy.
  • 19. Division of Medical Sciences, National Cancer Centre Singapore, Singapore.
  • 20. Cancer & Stem Cell Biology, Duke-NUS Medical School, Singapore.
  • 21. Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome, Italy.
  • 22. Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.
  • 23. Istituto Pasteur - Fondazione Cenci Bolognetti, Rome, Italy.
  • 24. Institute of Immunology, Hannover Medical School, Hannover, Germany.
  • 25. IRCCS Fondazione Santa Lucia, Rome, Italy.
  • 26. Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
  • 27. Center for Comparative Medicine & Dept. Pathology, Microbiology & Immunology, University of California, Davis, CA, USA.
  • 28. Institute for Immunology, Faculty of Medicine, Biomedical Center, LMU Munich, Planegg-Martinsried, Germany.
  • 29. Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
  • 30. Discipline of Pharmacy, Graduate School of Health, The University of Technology Sydney, Sydney, NSW, Australia.
  • 31. Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
  • 32. Comprehensive Cancer Center Zurich, Switzerland.
  • 33. Flow Cytometry Laboratory, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München, German Research Center for Environmental Health, München, Germany.
  • 34. Department of Biomedical Molecular Biology, Center for Inflammation Research, Ghent University - VIB, Ghent, Belgium.
  • 35. Flow Cytometry Core Technologies, UCD Conway Institute, University College Dublin, Dublin, Ireland.
  • 36. Department of Surgery, The University of British Columbia, Vancouver, Canada.
  • 37. BC Children's Hospital Research Institute, Vancouver, Canada.
  • 38. Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Erlangen, Germany.
  • 39. Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Medical Immunology Campus Erlangen, Erlangen, Germany.
  • 40. Department of Immunology and Pathology, Monash University, Melbourne, Victoria, Australia.
  • 41. Neuroimmunology and Flow Cytometry Units, Fondazione Santa Lucia IRCCS, Rome, Italy.
  • 42. Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA.
  • 43. The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Bronx, New York, USA.
  • 44. Thermo Fisher Scientific, Eugene, Oregon, USA.
  • 45. Luxembourg Institute of Health, Department of Infection and Immunity, Experimental and Molecular Immunology, Esch-sur-Alzette, Luxembourg.
  • 46. Odense University Hospital, Odense Research Center for Anaphylaxis, University of Southern Denmark, Department of Dermatology and Allergy Center, Odense, Denmark.
  • 47. Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Belvaux, Luxembourg.
  • 48. Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada.
  • 49. Terry Fox Laboratory, BC Cancer, Vancouver, BC, Canada.
  • 50. University of Auckland, School of Biological Sciences, Maurice Wilkins Center, Auckland, New Zealand.
  • 51. German Center for Infection Research (DZIF), Munich, Germany.
  • 52. Focus Group ''Clinical Cell Processing and Purification", Institute for Advanced Study, Technische Universität München, Munich, Germany.
  • 53. Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
  • 54. Department of Pediatrics and Shared Resource Laboratories, University of Rochester Medical Center, Rochester, NY, USA.
  • 55. University of Verona, Department of Medicine, Section of General Pathology, Verona, Italy.
  • 56. Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia.
  • 57. National Key Laboratory of Medical Immunology, Nankai University, Tianjin, China.
  • 58. Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden.
  • 59. The FIRC Institute of Molecular Oncology (FOM), Milan, Italy.
  • 60. National Institute for Health, Migration and Poverty (INMP), Rome, Italy.
  • 61. Macrophage Biology Group, School of Biology, University of Barcelona, Barcelona, Spain.
  • 62. Université Paris Descartes, Institut National de la Santé et de la Recherche Médicale, Paris, France.
  • 63. Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center, New York, USA.
  • 64. Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, Ontario, Canada.
  • 65. Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, UK.
  • 66. National Institutes of Health Research Biomedical Research Centre at Guy's and St. Thomas' National Health Service, Foundation Trust and King's College London, UK.
  • 67. Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
  • 68. IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.
  • 69. Department of Physiopathology and Transplants, University of Milan, Milan, Italy.
  • 70. Milan Center for Neuroscience, University of Milano-Bicocca, Milan, Italy.
  • 71. Department of Medicine, The University of British Columbia, Vancouver, Canada.
  • 72. Department of Pathology, University of Cambridge, Cambridge, UK.
  • 73. Department of Respiratory Sciences, University of Leicester, Leicester, UK.
  • 74. National Cytometry Platform, Luxembourg Institute of Health, Department of Infection and Immunity, Esch-sur-Alzette, Luxembourg.
  • 75. de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
  • 76. Unit Lymphopoiesis, Department of Immunology, Institut Pasteur, Paris, France.
  • 77. Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
  • 78. Clinical Research Unit, Berlin Institute of Health (BIH), Charite Universitätsmedizin Berlin, Berlin, Germany.
  • 79. Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
  • 80. Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, Victoria, Australia.
  • 81. Flow Cytometry Scientific Technology Platform, The Francis Crick Institute, London, UK.
  • 82. Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
  • 83. IRCCS Istituto Giannina Gaslini, Genova, Italy.
  • 84. Novo Nordisk Foundation Center for Stem Cell Biology - DanStem, University of Copenhagen, Copenhagen, Denmark.
  • 85. Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.
  • 86. Chair for Immunology, University Regensburg, Germany.
  • 87. Department of Medical Biotechnologies and Translational Medicine, University of Milan, Milan, Italy.
  • 88. Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
  • 89. Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey.
  • 90. Sony Europe Ltd, Weybridge, UK.
  • 91. Innate Immunty Unit, Department of Immunology, Institut Pasteur, Paris, France.
  • 92. Institut Pasteur, Inserm U1223, Paris, France.
  • 93. Charité - Universitätsmedizin Berlin, Laboratory of Innate Immunity, Department of Microbiology, Infectious Diseases and Immunology, Berlin, Germany.
  • 94. Berlin Institute of Health (BIH), Berlin, Germany.
  • 95. University of Palermo, Central Laboratory of Advanced Diagnosis and Biomedical Research, Department of Biomedicine, Neurosciences and Advanced Diagnostics, Palermo, Italy.
  • 96. Flow Cytometry Core Facility, Institute of Experimental Immunology, University of Bonn, Bonn, Germany.
  • 97. Dept. Medicine/Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Germany.
  • 98. Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
  • 99. Department of Dermatology, Laboratory of Dendritic Cell Biology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), University Hospital Erlangen, Erlangen, Germany.
  • 100. Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK.
  • 101. Program in Emerging Infectious Disease, Duke-NUS Medical School, Singapore.
  • 102. Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany.
  • 103. Department of Internal Medicine III, University Hospital Regensburg, Germany.
  • 104. Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Germany.
  • 105. Department of Immunology, University of Toronto, Toronto, ON, Canada.
  • 106. Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany.
  • 107. University of Barcelona, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences, Barcelona, Spain.
  • 108. Theodor Kocher Institute, University of Bern, Bern, Switzerland.
  • 109. Department of Immunology, University L. Eotvos, Budapest, Hungary.
  • 110. Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany.
  • 111. Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
  • 112. Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany.
  • 113. Division of Oncology, Washington University School of Medicine, St. Louis, MO, USA.
  • 114. Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Principe Felipe Research Center, Valencia, Spain.
  • 115. Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
  • 116. The Flow Cytometry Core Facility, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.
  • 117. MDQuest Ltd., Szeged, Hungary.
  • 118. Institut Necker-Enfants Malades, Université Paris Descartes Sorbonne Paris Cité, Faculté de Médecine, AP-HP, Hôpital Necker Enfants Malades, INSERM U1151-CNRS UMR 8253, Paris, France.
  • 119. Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • 120. Universitaetsklinikum Freiburg, Lighthouse Core Facility, Zentrum für Translationale Zellforschung, Klinik für Innere Medizin I, Freiburg, Germany.
  • 121. Immunology and Environment, LIMES Institute, University of Bonn, Bonn, Germany.
  • 122. Owl Biomedical Inc., Santa Barbara, USA.
  • 123. John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK.
  • 124. Department of Medicine, Albert Einstein College of Medicine, Bronx, NY, USA.
  • 125. Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venereology and Allergology.
  • 126. Nanobiophotonics Department, Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.
  • 127. School of Plant Sciences and Bio5 Institute, University of Arizona, Tucson, USA.
  • 128. Honorary Dean of Life Sciences, Henan University, Kaifeng, China.
  • 129. Division of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Amsterdam, The Netherlands.
  • 130. Institute of Experimental Immunology, University of Bonn, Germany.
  • 131. Stanford Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, CA, USA.
  • 132. Fundação Oswaldo Cruz - Minas, Laboratory of Immunopatology, Belo Horizonte, MG, Brazil.
  • 133. Department of Mecicine, University of Massachusetts Medical School, Worcester, MA, USA.
  • 134. INGM - Fondazione Istituto Nazionale di Genetica Molecolare "Ronmeo ed Enrica Invernizzi", Milan, Italy.
  • 135. Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
  • 136. Christian Doppler Laboratory for Optimized Prediction of Vaccination Success in Pigs, Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
  • 137. Department of Dermatology, Venereology and Allergology, Charité - Universitätsmedizin Berlin, Berlin, Germany.
  • 138. Translational Immunology Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore.
  • 139. Shanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • 140. Department of Bioengineering, University of California, Los Angeles, California, USA.
  • 141. Department of Chemistry, University of Tokyo, Tokyo, Japan.
  • 142. Institute of Technological Sciences, Wuhan University, Wuhan, China.
  • 143. Institut für Genetik, Universität zu Köln, Köln, Germany.
  • 144. Alicante Institute for Health and Biomedical Research (ISABIAL), Alicante, Spain.
  • 145. Networked Biomedical Research Center for Hepatic and Digestive Diseases (CIBERehd), Madrid, Spain.
  • 146. Institute of Infection Immunity and Inflammation, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, UK.
  • 147. Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, University of Milan.
  • 148. Cancer Immunology Research, Genentech, South San Francisco, CA, USA.
  • 149. Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.
  • 150. Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Zwijnaarde, Belgium.
  • 151. German Cancer Research Center (DKFZ), Heidelberg, Germany.
  • 152. German Cancer Consortium (DKTK), partner site Berlin, Berlin, Germany.
  • 153. Department of Hematology, Oncology, and Tumor Immunology, Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany.
  • 154. Department of Medicine and Center for Molecular Medicine at Karolinska University Hospital, Solna, Sweden.
  • 155. Murdoch Children's Research Institute, Parkville, Victoria, Australia.
  • 156. Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin.
  • 157. Department of Rheumatology and Clinical Immunology, Berlin Institute of Health, Berlin, Germany.
  • 158. Flow Cytometry, Houston Methodist Hospital Research Institute, Houston, TX, USA.
  • 159. Charité - Universitätsmedizin Berlin, Medical Department I, Division of Gastroenterology, Infectiology and Rheumatology, Berlin, Germany.
  • 160. Cytometry Service, Incliva Foundation. Clinic Hospital and Faculty of Medicine, University of Valencia, Valencia, Spain.
  • 161. Immunobiology Laboratory, Department of Biomedicine, University and University Hospital Basel, Basel, Switzerland.
  • 162. Cambridge Institute of Therapeutic Immunology & Infectious Disease, Jeffrey Cheah Biomedical Centre, University of Cambridge, Cambridge, UK.
  • 163. German Cancer Research Center (DKFZ), Division of Theoretical Systems Biology, Heidelberg, Germany.
  • 164. Center for Immunology, University of Minnesota, Minneapolis, MN, USA.
  • 165. Leiden Computational Biology Center, Leiden University Medical Center, Leiden, The Netherlands.
  • 166. Computer Graphics and Visualization, Department of Intelligent Systems, TU Delft, Delft, The Netherlands.
  • 167. Karolinska Institutet Biomedicum, Solna, Sweden.
  • 168. Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
  • 169. Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
  • 170. Department of Chemical & Materials Engineering, New Mexico State University, Las Cruces, NM, USA.
  • 171. Rheumatologie/Klinische Immunologie, Klinik für Innere Medizin I und Exzellenzzentrum Entzündungsmedizin, Universitätsklinikum Schleswig-Holstein, Kiel, Germany.
  • 172. Department of Immunology & National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China.
  • 173. Institute for Advanced Study (IAS), Shenzhen University, Shenzhen, China.
  • 174. Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
  • 175. Department of Hematology, Oncology and Rheumatology, University Heidelberg, Heidelberg, Germany.
  • 176. Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
  • 177. Department of Hematology, Singapore General Hospital, Singapore.
  • 178. Executive Offices, National Cancer Centre Singapore, Singapore.
  • 179. Department of Diagnostic Medicine, Clinical and Public Health, Univ. of Modena and Reggio Emilia, Modena, Italy.
  • 180. Max Planck Institute for Infection Biology, Berlin, Germany.
  • 181. Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain.
  • 182. Department of Histology and Embryology/Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
  • 183. Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic.
  • 184. Jena University Hospital, Institute of Immunology, Jena, Germany.
  • 185. Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • 186. Center for Chronic Immunodeficiency, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
  • 187. Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
  • 188. Institute of Molecular Health Sciences, ETH Zurich, Zürich, Switzerland.
  • 189. Department of Neuropathology, Universitätsklinikum Erlangen, Germany.
  • 190. John W Deming Endowed Chair in Internal Medicine, Center for Translational Research in Infection and Inflammation Tulane School of Medicine, New Orleans, LA, USA.
  • 191. Department of Neurology, Technical University of Munich, Munich, Germany.
  • 192. Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.
  • 193. Division of Cell Biology, Histology & Embryology, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria.
  • 194. Institute for Molecular Medicine, Goethe University Frankfurt, Frankfurt am Main, Germany.
  • 195. FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany.
  • 196. Flow & Mass Cytometry Core Facility, Charité - Universitätsmedizin Berlin and Berlin Institute of Health, Berlin, Germany.
  • 197. BCRT Flow Cytometry Lab, Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin.
  • 198. WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan.
  • 199. School of Biological Sciences, Nanyang Technological University, Singapore.
  • 200. INSERM U932, PSL University, Institut Curie, Paris, France.
  • 201. Department of Medicine and Aging Sciences, Centre on Aging Sciences and Translational Medicine (Ce.S.I.-Me.T.), University "G. d'Annunzio" of Chieti-Pescara, Chieti, Italy.
  • 202. Institut Cochin, CNRS8104, INSERM1016, Department of Endocrinology, Metabolism and Diabetes, Université de Paris, Paris, France.
  • 203. Section of Immunology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
  • 204. The Human Immune Monitoring Center (HIMC), Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, CA, USA.
  • 205. School of Biomedical Engineering, The University of British Columbia, Vancouver, Canada.
  • 206. Caprion Biosciences, ImmuneCarta, Montréal, Quebec, Canada.
  • 207. Center for Infectious Medicine, Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, Stockholm, Sweden.
  • 208. Inst. f. Med. Mikrobiology and Hospital Hygiene, University of Marburg, Germany.
  • 209. King's College London, "Peter Gorer" Department of Immunobiology, London, UK.
  • 210. Beckman Coulter, Inc, Miami, FL, USA.
  • 211. IMIM(Hospital de Mar Medical Research Institute), University Pompeu Fabra, Barcelona, Spain.
  • 212. Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH, USA.
  • 213. Department of Rheumatology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
  • 214. Centre d'Immunophénomique - CIPHE (PHENOMIN), Aix Marseille Université (UMS3367), Inserm (US012), CNRS (UMS3367), Marseille, France.
  • 215. Institute of Immunobiology, Kantonsspital St.Gallen, St. Gallen, Switzerland.
  • 216. Department of Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
  • 217. Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
  • 218. Flow and Image Cytometry Shared Resource, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.
  • 219. Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA, USA.
  • 220. Istituto Clinico Humanitas IRCCS and Humanitas University, Pieve Emanuele, Milan, Italy.
  • 221. William Harvey Research Institute, Queen Mary University, London, United Kingdom.
  • 222. Department of Immunology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.
  • 223. Cytomics Technological Service, Principe Felipe Research Center, Valencia, Spain.
  • 224. National Institute of Geriatrics, Rheumatology and Rehabilitation, Department of Pathophysiology and Immunology, Warsaw, Poland.
  • 225. Treg Cell Lab, Dipartimento di Medicina Molecolare e Biotecologie Mediche, Università di Napoli Federico II and Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), Napoli, Italy.
  • 226. Lab of Clinical and Experimental Immunology, Humanitas Clinical and Research Center, Rozzano, Milan, Italy.
  • 227. Cell Clearance in Health and Disease Lab, VIB Center for Inflammation Research, Ghent, Belgium.
  • 228. Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
  • 229. Ramaciotti Facility for Human Systems Biology, and Discipline of Pathology, The University of Sydney, Camperdown, Australia.
  • 230. Clinical Trial Center Leipzig, University Leipzig, Leipzig, Germany.
  • 231. Interdepartmental Immunobiology Center, Dept. of Microbiology-Immunology, Northwestern Univ. Medical School, Chicago, IL, USA.
  • 232. Trinity College Dublin, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Dublin, Ireland.
  • 233. Department of Clinical and Experimental Medine, Linköping University, Linköping, Sweden.
  • 234. Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
  • 235. Department of Immunology, IRCCS Bambino Gesu Children's Hospital, Rome, Italy.
  • 236. David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
  • 237. Centre for Environmental Research - UFZ, Department Environmental Microbiology, Leipzig, Germany.
  • 238. Institute for Innovative Radiotherapy (iRT), Experimental Immune Biology, Helmholtz Zentrum München, Neuherberg, Germany.
  • 239. Radiation Immuno-Oncology Group, Center for Translational Cancer Research Technische Universität München (TranslaTUM), Klinikum rechts der Isar, Munich, Germany.
  • 240. Department of Immunology, Graduate School of Medicine, Chiba University, Chiba city, Chiba, Japan.
  • 241. Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
  • 242. Discipline of Dermatology, University of Sydney, Sydney, New South Wales, Australia.
  • 243. State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.
  • 244. Barts and The London School of Medicine and Dentistry, Queen Mary University of London, UK.
  • 245. Department of Pathology and Rogel Cancer Center, the University of Michigan, Ann Arbor, Michigan, USA.
  • 246. Flow Cytometry Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
  • 247. Department of Medicine, Cancer Research Centre (IBMCC-CSIC/USAL), Cytometry Service, University of Salamanca, CIBERONC and Institute for Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.
  • 248. Burnet Institute, AMREP Flow Cytometry Core Facility, Melbourne, Victoria, Australia.
  • 249. Inflammation and Oncology, Research, Amgen Inc, South San Francisco, USA.
  • 250. ETH Zurich, Institute of Microbiology, Zurich, Switzerland.
  • 251. Department of Transfusion Medicine, Institute of Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany.
  • 252. Center of Excellence for Flow Cytometry, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
  • 253. Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
  • 254. Functional Cytomics Group, Josep Carreras Leukaemia Research Institute, Campus ICO-Germans Trias i Pujol, Universitat Autònoma de Barcelona, UAB, Badalona, Spain.
  • 255. Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
  • 256. Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
  • 257. Chromocyte Limited, Electric Works, Sheffield, UK.
  • 258. Department for Internal Medicine 3, Institute for Rheumatology and Immunology, AG Munoz, Universitätsklinikum Erlangen, Erlangen, Germany.
  • 259. Department of Immunology, University of Udine, Udine, Italy.
  • 260. School of Biomedical and Health Sciences, RMIT University, Bundoora, Victoria, Australia.
  • 261. Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
  • 262. Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
  • 263. Regeneration in Hematopoiesis, Leibniz-Institute on Aging, Fritz-Lipmann-Institute (FLI), Jena, Germany.
  • 264. Preparative Flow Cytometry, Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany.
  • 265. Bindley Biosciences Center, Purdue University, West Lafayette, IN, USA.
  • 266. Department of Internal Medicine, Groene Hart Hospital, Gouda, The Netherlands.
  • 267. Desatoya LLC, Reno, NV, USA.
  • 268. Immunobiology Laboratory, The Francis Crick Institute, London, UK.
  • 269. Renal Transplant Unit, Division of Internal Medicine, Academic Medical Centre, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
  • 270. Core Facility Flow Cytometry, Biomedical Center, Ludwig-Maximilians-University Munich, Germany.
  • 271. Department of Medical Microbiology and Immunology, University of Alberta, Alberta, Canada.
  • 272. Purdue University Cytometry Laboratories, Purdue University, West Lafayette, IN, USA.
  • 273. Cell Biology Unit, IRCCS Ospedale Policlinico San Martino, Genova, Italy.
  • 274. Institut für Klinische Chemie und Pathobiochemie, Fakultät für Medizin, Technische Universität München, München, Germany.
  • 275. Data Mining and Modeling for Biomedicine, VIB-UGent Center for Inflammation Research, Ghent, Belgium.
  • 276. Department of Applied Mathematics, Computer Science and Statistics, Ghent University, Ghent, Belgium.
  • 277. RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
  • 278. Flow Cytometry Facility, Ludwig Cancer Institute, Faculty of Medicine and Biology, University of Lausanne, Epalinges, Switzerland.
  • 279. Heidelberg University, Institute of Immunology, Section of Molecular Immunology, Heidelberg, Germany.
  • 280. Translational Immunology Laboratory, NIHR BRC, University of Oxford, Kennedy Institute of Rheumatology, Oxford, UK.
  • 281. Department of Molecular Medicine, Sapienza University of Rome, IRCCS, Neuromed, Pozzilli, Italy.
  • 282. Centre de Recherches des Cordeliers, Equipe Cancer et Immunité anti-tumorale, Paris, France.
  • 283. Charité - Universitätsmedizin Berlin, and Berlin Institute of Health, Institute of Medical Immunology, Berlin, Germany.
  • 284. Clinic for Orthopedics and Trauma Surgery, University Hospital Bonn, Bonn, Germany.
  • 285. R&D Reagents, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
  • 286. Quantitative Systems Biology, Life & Medical Sciences Institute, University of Bonn, Bonn, Germany.
  • 287. Internal Medicine I, University Hospital Regensburg, Germany.
  • 288. Flow Cytometry Core Facility, German Cancer Research Centre (DKFZ), Heidelberg, Germany.
  • 289. Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
  • 290. Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, Germany.
  • 291. University of Cambridge, Cambridge Institute for Medical Research, Cambridge, UK.
  • 292. Institut Pasteur, Cellular Lymphocytes Biology, Immunology Departement, Paris, France.
  • 293. TRI Flow Cytometry Suite (TRI.fcs), Translational Research Institute, Wooloongabba, QLD, Australia.
  • 294. AsedaSciences, West Lafayette, IN, USA.
  • 295. Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Portugal.
  • 296. Dept. Molecular Translational Medicine, University of Brescia, Brescia, Italy.
  • 297. Department of Oncology, University of Lausanne and CHUV, Epalinges, Switzerland.
  • 298. BD Life Sciences, Ashland, OR, USA.
  • 299. Lundberg Laboratory for Cancer, Department of Pathology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
  • 300. BD Life Sciences, San Diego, CA, USA.
  • 301. Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
  • 302. National Institutes of Health, Bethesda, MD, USA.
  • 303. Departement for Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.
  • 304. Institute for Medical Informatics, Statistics and Epidemiology (IMISE), University of Leipzig, Leipzig, Germany.
  • 305. Department of Precision Instruments, Tsinghua University, Beijing, China.
  • 306. BioGenes GmbH, Berlin, Germany.
  • 307. Novartis Biologics Center, Mechanistic Immunology Unit, Novartis Institute for Biomedical Research, NIBR, Basel, Switzerland.
  • 308. Department of Cellular and Molecular Physiology, Penn State University College of Medicine, PA, United States.
  • 309. Institute of Bioinnovation, BSRC Alexander Fleming, Vari, Greece.
  • 310. Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP, Brazil.
  • 311. Christine Kühne Center for Allergy Research and Education (CK-CARE), Davos, Switzerland.
  • 312. Vesicle Observation Center, Biomedical Engineering & Physics, Laboratory Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, The Netherlands.
  • 313. VIB Flow Core, VIB Center for Inflammation Research, Ghent, Belgium.
  • 314. Department of Infection Biology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Erlangen, Germany.
  • 315. BIH Center for Regenerative Therapies (BCRT) Charité Universitätsmedizin Berlin and Berlin Institute of Health, Core Unit ImmunoCheck.
  • 316. A·P·E-Applied Physics & Electronics, Berlin, Germany.
  • 317. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany.
  • 318. Flow Cytometry Facility, Babraham Institute, Cambridge, UK.
  • 319. Roswell Park Comprehensive Cancer Center, Elm and Carlton Streets, Buffalo, NY, USA.
  • 320. Dept of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
  • 321. The Scientific Platforms, the Westmead Institute for Medical Research, the Westmead Research Hub, Westmead, New South Wales, Australia.
  • 322. Flow Cytometry Core Facility, Blizard Institute, Queen Mary London University, London, UK.
  • 323. Faculty of Biological Sciences, Friedrich Schiller University Jena, Jena, Germany.
  • 324. Medinfomatics Ltd., West Drayton, Middlesex, UK.
  • 325. Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund (IfADo), Dortmund, Germany.
  • 326. Institute for Cellular & Molecular Immunology, University Medical Center Göttingen, Göttingen, Germany.
  • 327. Department of Infectious Disease, Imperial College London, UK.
  • 328. Wellcome Centre for Infectious Diseases Research in Africa and Department of Medicine, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Republic of South Africa.
  • 329. Tuberculosis Laboratory, The Francis Crick Institute, London, UK.
  • 330. Cooperation Unit for Experimental and Translational Cancer Immunology, Institute of Immunology (Charité - Universitätsmedizin Berlin) and German Cancer Research Center (DKFZ), Berlin, Germany.
  • 331. University Bonn, Medical Faculty, Bonn, Germany.
  • 332. BD Biosciences, San Jose, CA, USA.
  • 333. School of Computing Science, Simon Fraser University, Burnaby, Canada.
  • 334. Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
  • 335. Institute of Virology, Technical University of Munich, Munich, Germany.
  • 336. TranslaTUM, Technical University of Munich, Munich, Germany.
  • 337. Maurice Müller Laboratories (Department of Biomedical Research), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland.
PMID: 31633216 DOI: 10.1002/eji.201970107
Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.