Endothelial Yes-Associated Protein 1 Promotes Astrocyte Proliferation and Maturation via Cytoplasmic Leukemia Inhibitory Factor Secretion in Oxygen-Induced Retinopathy

  • Invest Ophthalmol Vis Sci. 2020 Apr 9;61(4):1. doi: 10.1167/iovs.61.4.1.
Li-Qian-Yu Ai  1 ,  Jing-Yi Zhu  1 ,  Xi Chen  1 ,  Xue Li  1 ,  Lin-Lin Luo  1 ,  Qiu-Mei Hu  1 ,  Sen Lin  1 ,  Jian Ye  1
Affiliations
  • 1. ,.
PMID: 32271890 DOI: 10.1167/iovs.61.4.1
Abstract

Purpose: Purpose The role of endothelial Yes-associated protein 1 (YAP) in the pathogenesis of retinal angiogenesis and the astrocyte network in the mouse oxygen-induced retinopathy (OIR) model is unknown.

Methods: For in vivo studies, OIR was induced in conditional endothelial YAP knockout mice and their wild-type littermates. Retinal vascularization and the astrocyte network were evaluated by whole-mount fluorescence and Western blotting. In vitro experiments were performed in astrocytes cultured with human microvascular endothelial cell-1-conditioned medium to analyze the mechanisms underlying the effect of endothelial YAP on astrocytes.

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