Isolation and biological activity of agrostophillinol from kaffir lime (Citrus hystrix) leaves

  • Bioorg Med Chem Lett. 2020 Jul 15;30(14):127256. doi: 10.1016/j.bmcl.2020.127256.
Songyot Anuchapreeda  1 ,  Riki Anzawa  2 ,  Natsima Viriyaadhammaa  3 ,  Waranya Neimkhum  4 ,  Wantida Chaiyana  5 ,  Siriporn Okonogi  6 ,  Toyonobu Usuki  7
Affiliations
  • 1. Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan; Cancer Research Unit of Associated Medical Sciences (AMS CRU), Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: [email protected].
  • 2. Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan.
  • 3. Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
  • 4. Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Huachiew Chalermprakiet University, Samutprakarn 10250, Thailand.
  • 5. Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, Thailand.
  • 6. Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center of Pharmaceutical Nanotechnology, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: [email protected].
  • 7. Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, Tokyo 102-8554, Japan. Electronic address: [email protected].
PMID: 32527555 DOI: 10.1016/j.bmcl.2020.127256
Abstract

The leaves of the kaffir lime (Citrus hystrix) are commonly used in cuisine and folk medicine. The aim of this study was to isolate a bioactive compound in kaffir lime leaves and characterize its biological activity. The compound was isolated from a hexane fractional extract and identified as agrostophillinol. This is the first report of agrostophillinol isolated from kaffir lime leaves. In terms of cytotoxicity, agrostophillinol exhibited IC50 values of 36.27 ± 7.30 and 53.44 ± 10.63 μg/mL against EoL-1 and HL60 cells, respectively. Agrostophillinol also exhibited potent anti-inflammatory activity, significantly inhibiting IL-6 secretion.

Keywords
Agrostophillinol; Anti-inflammatory; Anticancer; Antioxidant; Citrus hystrix; Kaffir lime.