Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Ganoderma leucocontextum fruiting bodies

  • Carbohydr Polym. 2020 Dec 1;249:116874. doi: 10.1016/j.carbpol.2020.116874.
Xiong Gao  1 ,  Jiayi Qi  2 ,  Chi-Tang Ho  3 ,  Bin Li  2 ,  Jingjing Mu  4 ,  Yuting Zhang  2 ,  Huiping Hu  4 ,  Weipeng Mo  4 ,  Zhongzheng Chen  5 ,  Yizhen Xie  6
Affiliations
  • 1. State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China; Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, 510663, China.
  • 2. College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, 510642, China.
  • 3. Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ, 08901, USA.
  • 4. State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China.
  • 5. College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, 510642, China. Electronic address: [email protected].
  • 6. State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, China; Guangdong Yuewei Edible Fungi Technology Co. Ltd., Guangzhou, 510663, China. Electronic address: [email protected].
PMID: 32933694 DOI: 10.1016/j.carbpol.2020.116874
Abstract

Ganoderma leucocontextum is a new species of Ganoderma discovered in 2014. Up to now, the structural characteristics and immunoregulatory activity of its Polysaccharides remain virtually unknown. In this study, a water-soluble polysaccharide termed, GLP-3, was purified from G. leucocontextum by ultrafiltration and column chromatography. The results revealed that GLP-3 mainly consisted of glucose (92.7 %) and its weight average molecular weight was 159.7 kDa. The structural analysis indicated that the backbone of GLP-3 was →4)-α-D-Glcp-(1→4,6)-β-D-Glcp-(1→ with a β-Glcp-(1→ branch. Atomic force microscopy and Congo red experiments revealed that GLP-3 might possess a globular structure with triple-helix conformation in water. Moreover, GLP-3 was recognized by Toll-like Receptor 2 (TLR2) and exerted immunomodulatory effects via activating mitogen-activated protein kinases (MAPKs), phosphatidylinositol-3-kinase (PI3K)/Akt and nuclear factor-κB (NF-κB) signaling pathways in RAW 264.7 macrophages. Collectively, these results suggested that GLP-3 could be developed as a potential functional food ingredient for immunomodulation.

Keywords
Ganoderma leucocontextum; Immunostimulatory activity; Polysaccharide; Structure; Toll-like receptor 2.
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