Tools of the trade: studying actin in zebrafish

  • Histochem Cell Biol. 2020 Nov;154(5):481-493. doi: 10.1007/s00418-020-01932-3.
Clyde Savio Pinto  1 ,  Masanori Mishima  2  3 ,  Karuna Sampath  4  5  6
Affiliations
  • 1. Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK. [email protected].
  • 2. Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK.
  • 3. Centre for Mechanochemical Cell Biology, University of Warwick, Coventry, CV4 7AL, UK.
  • 4. Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK. [email protected].
  • 5. Centre for Mechanochemical Cell Biology, University of Warwick, Coventry, CV4 7AL, UK. [email protected].
  • 6. Center for Early Life, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK. [email protected].
PMID: 33095903 DOI: 10.1007/s00418-020-01932-3
Abstract

Actin is a conserved cytoskeletal protein with essential functions. Here, we review the state-of-the-art reagents, tools and methods used to probe actin biology and functions in zebrafish embryo and larvae. We also discuss specific cell types and tissues where the study of actin in zebrafish has provided new insights into its functions.

Keywords
Actin; Cytoskeleton; Imaging; Inhibitors; Zebrafish.