The role of STIM1/ORAI1 channel in the analgesic effect of grain-sized moxibustion on inflammatory pain mice model

  • Life Sci. 2021 Sep 1;280:119699. doi: 10.1016/j.lfs.2021.119699.
Cheng-Shun Zhang  1 ,  Chuan-Yi Zuo  2 ,  Peng Lv  1 ,  Han-Xiao Zhang  1 ,  Si-Rui Lin  3 ,  Rui-Zhen Huang  4 ,  Gang Shi  5 ,  Xiao-Qin Dai  6
Affiliations
  • 1. Acupuncture and Tuina School/Third Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China.
  • 2. Department of Acupuncture, Chongqing Traditional Chinese Medicine Hospital, Chongqing 400021, China.
  • 3. Department of Acupuncture, Southwest Medical University Affiliated Traditional Chinese Medicine Hospital, Luzhou 646000, Sichuan Province, China.
  • 4. Department of Pharmacy Service, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China.
  • 5. Department of Pharmacy Service, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, Sichuan Province, China. Electronic address: [email protected].
  • 6. Department of Traditional Chinese Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 611731, Sichuan Province, China; Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu 610072, Sichuan Province, China. Electronic address: [email protected].
PMID: 34102196 DOI: 10.1016/j.lfs.2021.119699
Abstract

The therapeutic effect of grain-sized moxibustion (GS-Moxi) on inflammatory pain has been well recognized clinically, but the mechanism remains unclear. STIM1/ORAI1 is a sensible temperature channel, therefore; this study aimed to investigate the analgesic effect of GS-Moxi and the association with STIM1/ORAI1 expression. CFA-induced inflammatory pain model was established and was treated with GS-Moxi after 3 days of CFA injection. The behavioral test was measured after the GS-Moxi; then, serum was prepared for IL-1β, IL-6, and TNF-α, and the stimulated skin was used for measuring STIM1 and ORAI1 expression. The results indicated GS-Moxi had an analgesic effect on inflammatory pain and the heat variation was significant for the analgesia. GS-Moxi decreased the expression of IL-1β, IL-6, and TNF-α. Immunofluorescence and western blot analysis illustrated that heat change was associated with the stimulation of STIM1 and ORAI1. Suggesting that heat variation created by GS-Moxi could be crucial in this therapy and STIM1 and ORAI1 were potential enhancers in regulating analgesia of GS-Moxi.

Keywords
Analgesia; Grain-sized moxibustion; Inflammatory pain; ORAI1; STIM1.
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