In situ label-free and sensitive detection assay for cell apoptosis via polyadenosine-coralyne fluorescence enhancement strategy

  • Anal Biochem. 2021 Nov 1;632:114329. doi: 10.1016/j.ab.2021.114329.
Chun Wu  1 ,  Jingru Wang  1 ,  Yihang Chen  1 ,  Xiwen Xing  2
Affiliations
  • 1. Department of Biotechnology, College of Life Science and Technology, Jinan University Guangzhou, 510632, China.
  • 2. Department of Biotechnology, College of Life Science and Technology, Jinan University Guangzhou, 510632, China. Electronic address: [email protected].
PMID: 34525387 DOI: 10.1016/j.ab.2021.114329
Abstract

Cell Apoptosis detection is vital for biological analysis and clinical application; some detection assays are already commercially available. However, it is still far from perfect and needs further improvement for less cost, time-consuming and operation demanding. TUNEL, a high market share cell Apoptosis assay, depends on adulteration fluorescent labelling dUTP by terminal deoxynucleotidyl transferase(TdT) which randomly adds deoxyribonucleoside triphosphates (dNTPs) at the 3'-OH terminal of ssDNA with a template-free manner. Based on our previous work, we adopted a label-free strategy to reduce the cost and operation maintenance of TUNEL and developed a facile, rapid, convenient and in-situ assay for cell Apoptosis.

Keywords
Apoptosis; Coralyne; Fluorescence enhancement; Polyadenosine; Terminal deoxyribonuclease transferase.
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