Bioactive Properties of a Novel Antibacterial Dye Obtained from Laccase-Mediated Oxidation of 8-Anilino-1-naphthalenesulfonic Acid

  • Molecules. 2022 Jan 13;27(2):487. doi: 10.3390/molecules27020487.
Jolanta Polak  1 ,  Marcin Grąz  1 ,  Kamila Wlizło  2 ,  Katarzyna Szałapata  1 ,  Justyna Kapral-Piotrowska  3 ,  Roman Paduch  4 ,  Anna Jarosz-Wilkołazka  1
Affiliations
  • 1. Department of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, Poland.
  • 2. Department of Industrial and Environmental Microbiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, Poland.
  • 3. Department of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, Poland.
  • 4. Department of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 20-031 Lublin, Poland.
PMID: 35056804 DOI: 10.3390/molecules27020487
Abstract

Fungal laccase obtained from a Cerrena unicolor strain was used as an effective biocatalyst for the transformation of 8-anilino-1-naphthalenesulfonic acid into a green-coloured Antibacterial compound, which can be considered as both an antimicrobial agent and a textile dye, simultaneously. The process of biosynthesis was performed in buffered solutions containing methanol as a co-solvent, allowing better solubilisation of substrate. The transformation process was optimised in terms of the buffer pH value, laccase activity, and concentrations of the substrate and co-solvent. The crude product obtained exhibited low cytotoxicity, Antibacterial properties against Staphylococcus aureus and Staphylococcus epidermidis, and antioxidant properties. Moreover, the synthesised green-coloured compound proved non-allergenic and demonstrated a high efficiency of dyeing wool fibres.

Keywords
bioactive compounds; biocatalysis; laccase.
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