Genetic encoding of ε- N-L-lactyllysine for detecting delactylase activity in living cells

  • Chem Commun (Camb). 2022 Jul 28;58(61):8544-8547. doi: 10.1039/d2cc02643k.
Yanan Sun  1 ,  Yanchi Chen  1 ,  Yaxin Xu  1 ,  Yuqing Zhang  1 ,  Minghao Lu  1 ,  Manjia Li  1 ,  Liyan Zhou  1 ,  Tao Peng  1  2
Affiliations
  • 1. State Key Laboratory of Chemical Oncogenomics, Guangdong Provincial Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, 518055, China. [email protected].
  • 2. Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China.
PMID: 35815577 DOI: 10.1039/d2cc02643k
Abstract

Lysine ε-N-L-lactylation is a newly discovered post-translational modification. Herein we present the genetic encoding of ε-N-L-lactyllysine in Bacterial and mammalian cells, allowing the preparation of site-specifically ε-N-L-lactylated recombinant proteins and the construction of fluorescent and luminescent probes for detecting delactylases in living cells. Using these probes, we demonstrate Sirtuin 1 as a potential delactylase for non-histone proteins.

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