2-Methylimidazole-doped nanozymes with enhanced laccase activity for the (+)-catechins detection in dairy products
- Talanta. 2023 Jan 15:252:123853. doi: 10.1016/j.talanta.2022.123853.
- 1. College of Food Science and Engineering, Jilin University, Changchun, 130025, China.
- 2. College of Chemistry, Jilin University, Changchun, 130012, PR China.
- 3. College of Food Science and Engineering, Jilin University, Changchun, 130025, China. Electronic address: [email protected].
- 4. Key Lab of Groundwater Resources and Environment of Ministry of Education, Key Lab of Water Resources and Aquatic Environment of Jilin Province, College of New Energy and Environment, Jilin University, Changchun, 130021, PR China. Electronic address: [email protected].
In this work, 2-methylimidazole (MI) was doped into the Bpy-Cu nanozyme as a second ligand to form a novel laccase-mimicking enzyme (MI-Bpy-Cu). By comparison, MI-Bpy-Cu nanozyme was identified to have excellent laccase-mimicking activity, high stability and catalytic kinetic properties. It may be that the incorporation of 2-methylimidazole helped the nanozymes to build a structure closer to natural laccase and accelerate the electron transfer rate, thereby achieving the purpose of enhancing the activity. Furthermore, we observed that MI-Bpy-Cu nanozymes could oxidize the colorless (+)-Catechin to generate a yellow radical product, and a novel colorimetric sensing strategy for (+)-Catechin was successfully developed. The method had excellent selectivity and anti-interference properties, and also had good application in the analysis of (+)-Catechin in dairy products.