Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088

  • Methods Mol Biol. 2023:2566:345-353. doi: 10.1007/978-1-0716-2675-7_28.
Franco Cheli  1 ,  Sara Falsini  2 ,  Maria Cristina Salvatici  3 ,  Sandra Ristori  4 ,  Silvia Schiff  5 ,  Emilio Corti  5 ,  Irene Costantini  1 ,  Cristina Gonnelli  5 ,  Francesco Saverio Pavone  1 ,  Alessio Papini  5
Affiliations
  • 1. LENS - European Laboratory for Non-linear Spectroscopy, University of Florence, Florence, Italy.
  • 2. Department of Biology, University of Florence, Florence, Italy. [email protected].
  • 3. Institute of Chemistry of Organometallic Compounds (ICCOM)-Electron Microscopy Centre (Ce.M. E.), National Research Council (CNR), Florence, Italy.
  • 4. Department of Chemistry, University of Florence, Florence, Italy.
  • 5. Department of Biology, University of Florence, Florence, Italy.
PMID: 36152265 DOI: 10.1007/978-1-0716-2675-7_28
Abstract

The microscopic visualization of nanoparticles in Plants is crucial to elucidate the mechanisms of their uptake through the cell wall and plasma membrane and to localize the possible sites of their extracellular or intracellular accumulation. Lignin nanocarriers are polymeric hollow nanocapsules able to contain and transport several bioactive substances inside plant tissues. We describe here a method for the preparation of Fluorol Yellow 088-labeled lignin nanocapsules that allow their localization in plant organs and tissues by fluorescence microscopy.

Keywords
Confocal microscopy; Fluorescence microscopy; Lignin nanocarriers; Nanocapsules; Scanning electron microscopy; Two-photon microscopy.
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