A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells

  • STAR Protoc. 2022 Oct 26;3(4):101786. doi: 10.1016/j.xpro.2022.101786.
Tomoka Takao  1 ,  Daisuke Yamada  2 ,  Takeshi Takarada  3
Affiliations
  • 1. Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8582, Japan. Electronic address: [email protected].
  • 2. Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8582, Japan. Electronic address: [email protected].
  • 3. Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8582, Japan. Electronic address: [email protected].
PMID: 36317170 DOI: 10.1016/j.xpro.2022.101786
Abstract

Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1+ limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).

Keywords
Cell culture; Cell differentiation; Developmental biology; Organoids; Stem cells; Tissue engineering.
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