A natural allele of proteasome maturation factor improves rice resistance to multiple pathogens

  • Nat Plants. 2023 Jan 16. doi: 10.1038/s41477-022-01327-3.
Xiao-Hong Hu  #  1 ,  Shuai Shen  #  1 ,  Jin-Long Wu  #  1 ,  Jie Liu  #  1 ,  He Wang  #  1 ,  Jia-Xue He  1 ,  Zong-Lin Yao  1 ,  Yi-Fei Bai  1 ,  Xin Zhang  1 ,  Yong Zhu  1 ,  Guo-Bang Li  1 ,  Jing-Hao Zhao  1 ,  Xiaoman You  2 ,  Jie Xu  1  3 ,  Yun-Peng Ji  1 ,  De-Qiang Li  1 ,  Mei Pu  1 ,  Zhi-Xue Zhao  1 ,  Shi-Xin Zhou  1 ,  Ji-Wei Zhang  1 ,  Yan-Yan Huang  1 ,  Yan Li  1 ,  Yuese Ning  2 ,  Yanli Lu  1  3 ,  Fu Huang  1  4 ,  Wen-Ming Wang  5 ,  Jing Fan  6
Affiliations
  • 1. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China.
  • 2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
  • 3. Maize Research Institute, Sichuan Agricultural University, Chengdu, China.
  • 4. College of Agronomy, Sichuan Agricultural University, Chengdu, China.
  • 5. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. [email protected].
  • 6. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China. [email protected].
  • # Contributed equally.
PMID: 36646829 DOI: 10.1038/s41477-022-01327-3
Abstract

Crops with broad-spectrum resistance loci are highly desirable in agricultural production because these loci often confer resistance to most races of a pathogen or multiple pathogen species. Here we discover a natural allele of Proteasome maturation factor in rice, UMP1R2115, that confers broad-spectrum resistance to Magnaporthe oryzae, Rhizoctonia solani, Ustilaginoidea virens and Xanthomonas oryzae pv. oryzae. Mechanistically, this allele increases Proteasome abundance and activity to promote the degradation of reactive oxygen species-scavenging Enzymes including peroxidase and catalase upon pathogen Infection, leading to elevation of H2O2 accumulation for defence. In contrast, inhibition of Proteasome function or overexpression of peroxidase/catalase-encoding genes compromises UMP1R2115-mediated resistance. More importantly, introduction of UMP1R2115 into a disease-susceptible rice variety does not penalize grain yield while promoting disease resistance. Our work thus uncovers a broad-spectrum resistance pathway integrating de-repression of plant immunity and provides a valuable genetic resource for breeding high-yield rice with multi-disease resistance.

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