Proanthocyanidin oligomers extract from hawthorn mediates cell cycle arrest, apoptosis, and lysosome vacuolation on HCT116 cells

  • Curr Res Food Sci. 2022 May 29:5:904-917. doi: 10.1016/j.crfs.2022.05.009.
Ya-Sai Sun  1  2  3 ,  Zi-Wei Wang  1 ,  Zhe Gao  1 ,  Wen Zhao  1 ,  Kiran Thakur  2  3 ,  Qian Zhong  2  3 ,  Zhao-Jun Wei  2  3
Affiliations
  • 1. College of Food Science and Technology, Agricultural University of Hebei, Baoding, 071001, PR China.
  • 2. School of Biological Science and Engineering, Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, Yinchuan, 750021, PR China.
  • 3. School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, PR China.
PMID: 36686366 DOI: 10.1016/j.crfs.2022.05.009
Abstract

In this study, Hawthorn oligomic procyanidins extracts (HPOE) were evaluated for their Anticancer activity on colorectal Cancer. Our results showed that HPOE arrested HCT116 cells cycle at G2/M phase through P53-Cyclin B pathway and promoted Apoptosis partly via mitochondrial (Caspase 9-Caspase 3) and death receptor (Caspase 8-Caspase 3) pathways. Meanwhile, it was found that HPOE aggravated HCT116 cells death through lysosomal vacuolation, which was verified by inhibitor/activator of P53-ILC3 signaling pathway. Taken together, HPOE exerted Anticancer effects which lays the foundation for the development of functional foods for clinical colon Cancer patients.

Keywords
Apoptosis; Cell cycle; Colon cancer; Hawthorn proanthocyanidin oligomers extract; Lysosomes; Vacuolation.
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