A review of tamoxifen administration regimen optimization for Cre/loxp system in mouse bone study

  • Biomed Pharmacother. 2023 Sep:165:115045. doi: 10.1016/j.biopha.2023.115045.
Ming-Yang Chen  1 ,  Fu-Lin Zhao  1 ,  Wen-Lin Chu  1 ,  Ming-Ru Bai  2 ,  De-Mao Zhang  3
Affiliations
  • 1. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
  • 2. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Department of Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, China. Electronic address: [email protected].
  • 3. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Institute of Biomedical Engineering, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, China. Electronic address: [email protected].
PMID: 37379643 DOI: 10.1016/j.biopha.2023.115045
Abstract

Gene knockout is a technique routinely used in basic experimental research, particularly in mouse skeletal and developmental studies. Tamoxifen-induced Cre/loxp system is known for its temporal and spatial precision and commonly utilized by researchers. However, tamoxifen has been shown its side effects on affecting the phenotype of mouse bone directly. This review aimed to optimize tamoxifen administration regimens including its dosage and duration, to identify an optimal induction strategy that minimizes potential side effects while maintaining recombination efficacy. This study will help researchers in designing gene knockout experiments in bone when using tamoxifen.

Keywords
Bone metabolism; Cre/loxp system; Mouse bone; Tamoxifen administration; Tamoxifen-induced side effects.
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