Photothermal theranostics with glutathione depletion and enhanced reactive oxygen species generation for efficient antibacterial treatment

  • RSC Adv. 2023 Jul 28;13(33):22863-22874. doi: 10.1039/d3ra03246a.
Yuelan Wu  1  2 ,  Xiaoxue Liu  2  3 ,  Xiaoyu Zhang  2  3 ,  Shuping Zhang  3  4 ,  Panhong Niu  4 ,  Hua Gao  1  2  3
Affiliations
  • 1. Qingdao University Qingdao Shandong 266071 P. R. China [email protected].
  • 2. Eye Institute of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology Qingdao Shandong 250071 P. R. China.
  • 3. Medical Science and Technology Innovation Center, Shandong First Medical University & Shandong Academy of Medical Sciences Jinan Shandong 250117 P. R. China.
  • 4. Biomedical Sciences College & Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences Jinan 250117 P. R. China [email protected].
PMID: 37520103 DOI: 10.1039/d3ra03246a
Abstract

Drug-resistant bacteria caused by the abuse of Antibiotics have brought great challenges to antimicrobial therapy. Herein an antibiotic-free polydopamine (PDA) modified metal-organic framework (PDA-FDM-23) with photothermal-enhanced chemodynamic effect was developed for synergistic Antibacterial treatment. The PDA-FDM-23 Antibacterial agent exhibited high peroxidase-like activity. Moreover, the process was significantly accelerated by consuming glutathione (GSH) to generate more efficient oxidizing Cu+. In addition, the photothermal therapy (PTT) derived from PDA improved the chemodynamic therapy (CDT) activity triggering a Reactive Oxygen Species explosion. This PTT-enhanced CDT strategy illustrated 100% Antibacterial efficiency against both Staphylococcus aureus and Escherichia coli. Cytotoxicity and hemolysis analyses fully demonstrated the excellent biocompatibility of PDA-FDM-23. Overall, our work highlighted the strong peroxidase catalytic activity, excellent GSH consumption and photothermal performance of PDA-FDM-23, providing a new strategy for antibiotic-free Reactive Oxygen Species (ROS) synergistic sterilization.

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