Non-Targeted Metabolomics Combined with Chemometrics by UHPLC-Orbitrap-HRMS and Antioxidant Activity of Atractylodes chinensis (DC.) Koidez. from Eight Origins

  • Metabolites. 2023 Jul 27;13(8):888. doi: 10.3390/metabo13080888.
Xueyan Gao  1  2  3 ,  Danyang Ma  1  2  3 ,  Kaiyuan Li  1  2  3 ,  Tianjiao Xing  1  2  3 ,  Xiwu Liu  4 ,  Lingfeng Peng  1  2  3 ,  Dawei Chen  5 ,  Zhihui Hao  1  2  3
Affiliations
  • 1. Chinese Veterinary Medicine Innovation Center, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
  • 2. Key Biology Laboratory of Chinese Veterinary Medicine, Ministry of Agriculture and Rural Affairs, Beijing 100193, China.
  • 3. National Center of Technology Innovation for Medicinal Function of Food, National Food and Strategic Reserves Administration, Beijing 100193, China.
  • 4. Qingdao Animal Husbandry Workstation, Qingdao 266100, China.
  • 5. NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (No. 2019RU014), China National Center for Food Safety Risk Assessment, Beijing 100021, China.
PMID: 37623832 DOI: 10.3390/metabo13080888
Abstract

Atractylodes chinensis (DC.) Koidez. (AC) is a type of Atractylodis Rhizoma that is widely used in China to treat diarrhea and arthritis, as well as a nutritional supplement. The objective of this study was to investigate and identify the phytochemicals in the aqueous extract of AC using an ultra-high-performance liquid chromatography (UHPLC)-Orbitrap-HRMS platform based on a non-targeted metabolomic approach. There were 76 compounds in the AC, the majority of which were Phenylpropanoids (16) and Terpenoids (15). The hierarchical clustering analysis (HCA) and principal component analysis (PCA) results revealed variations across eight AC samples and classified them into four groups. Using Pareto modeling, the orthogonal partial least squares-discriminant analysis (OPLS-DA) identified 11 distinct AC compounds. Furthermore, the antioxidant activity of eight AC samples was assessed using ABTS, DPPH, and OH· methods. The AC samples with concentrations ranging from 0 to 25 mg/mL had no toxic effects on A549 cells. They have a strong therapeutic potential against oxidation-related diseases, and further research on AC is warranted.

Keywords
Atractylodes chinensis (DC.) Koidez.; antioxidant activity; chemometrics; non-targeted metabolomics; origin.
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