PI4KB is an essential host factor for duck hepatitis a virus 1 replication and translation

  • Int J Biol Macromol. 2024 Nov;281(Pt 3):136480. doi: 10.1016/j.ijbiomac.2024.136480.
Xinhong Li  1 ,  Mingshu Wang  1 ,  Anchun Cheng  2 ,  Wei Zhang  3 ,  Qiao Yang  1 ,  Bin Tian  1 ,  Xumin Ou  1 ,  Di Sun  1 ,  Yu He  1 ,  Xinxin Zhao  1 ,  Ying Wu  1 ,  Shaqiu Zhang  1 ,  Juan Huang  1 ,  Zhen Wu  1 ,  Yanling Yu  4 ,  Ling Zhang  4 ,  Dekang Zhu  5 ,  Renyong Jia  1 ,  Mafeng Liu  1 ,  Shun Chen  1
Affiliations
  • 1. Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
  • 2. Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: [email protected].
  • 3. Sinopharm Yangzhou VAC Biological Engineering Co., Ltd., Yangzhou 225100, China.
  • 4. Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China.
  • 5. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China.; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China.; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
PMID: 39393728 DOI: 10.1016/j.ijbiomac.2024.136480
Abstract

Duck hepatitis A virus 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. Phosphatidylinositol 4-kinases (PI4Ks) are important for viral replication, and different viruses have different strategies to hijack PI4Ks. To date, few studies have investigated the DHAV-1 life cycle; thus, whether PI4Ks are required for DHAV-1 replication has not been reported. In this study, we found that the PI4KB protein, a PI4K, promoted the replication and translation of DHAV-1, and the 2A2, 2C, 2BC, 3A, 3AB, 3D, and 3CD proteins of DHAV-1 were able to interact with the PI4KB protein. Amino acids 101-120 of the 2A2 protein is the region where the 2A2 protein interacts with the PI4KB protein.

Keywords
2A2 protein; Duck hepatitis A virus 1; Phosphatidylinositol 4-kinase beta protein.
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