Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate

  • Appl Environ Microbiol. 1982 Jul;44(1):238-41. doi: 10.1128/aem.44.1.238-241.1982.
A G Ostle ,  J G Holt
PMID: 6181737 DOI: 10.1128/aem.44.1.238-241.1982
Abstract

Poly-beta-hydroxybutyrate granules exhibited a strong orange fluorescence when stained with Nile blue A. Heat-fixed cells were treated with 1% Nile blue A for 10 min and were observed at an excitation wavelength of 460 nm. Glycogen and polyphosphate did not stain. Nile blue A appears to be a more specific stain for poly-beta-hydroxybutyrate than Sudan black B.

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