Amphistin, a new melanogenesis inhibitor, produced by an actinomycete

  • J Antibiot (Tokyo). 1997 Oct;50(10):808-14. doi: 10.7164/antibiotics.50.808.
N Arai  1 ,  K Shiomi ,  S Takamatsu ,  K Komiyama ,  M Shinose ,  Y Takahashi ,  Y Tanaka ,  Y Iwai ,  J R Liu ,  S Omura
Affiliations
  • 1. Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.
PMID: 9402984 DOI: 10.7164/antibiotics.50.808
Abstract

A new melanogenesis inhibitor, named amphistin, was isolated from the fermentation broth of an actinomycete strain KP-3052. Amphistin was purified from the culture filtrate by the combination of cation exchange, gel filtration, and aminosilyl silica gel chromatographic methods. The structure of amphistin was elucidated as gamma-(beta-histidinoalanino)homoalanine by NMR experiments including 1H-15N HMBC experiment and Other spectroscopic analyses. Amphistin inhibited the melanogenesis of B16 melanoma cells at concentration of 6.8 microM.

Products