Recombinant Kex2 protease
Based on 1 Customer Validation
Recombinant Kex2 protease is a membrane-bound, Ca2+-dependent serine protease. Recombinant Kex2 protease specifically recognize and cleave the carboxyl-terminal peptide bonds of dibasic amino acids.
For research use only. We do not sell to patients.
- CAS No.: 99676-46-7
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Protocol
1) Reaction buffer: 50 mM Tris-HCl, 2 mM CaCl2 (pH 7.0-9.0).
2) Storage buffer: 20 mM (pH 5.2) NaAc-HAc, 2 mM CaCl2. If not used immediately after dissolution, the product can be dissolved in storage buffer. The final concentration of the enzyme after dissolution is about 1-10mg/ml, according to It needs to be aliquoted and stored below -20°C.
3) Reaction conditions: The optimal reaction pH is 9.0, and the stable pH is 5.0-6.0.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
EC Number
3.4.21.61
Specific Activity
≥10 U/mg protein
Unit Definition
One unit is defined as the amount of enzyme that can catalyze substrate Boc-QRR-pNA to release 1 μmol 4-nitroaniline per min, in the 3 mL reaction system of 25°C, 50 mM Tris-HCl, 2 mM CaCl2, pH 8.
Chemical Information
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CAS No. 99676-46-7
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Appearance Solid
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Color White to off-white
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SMILES
[Recombinant Kex2 protease]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Purity & Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Recombinant Kex2 protease Related Classifications
HY-E70200 Related Classifications
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