Recombinant Kex2 protease
Based on 1 Customer Validation
Recombinant Kex2 protease is a membrane-bound, Ca2+-dependent serine protease. Recombinant Kex2 protease specifically recognize and cleave the carboxyl-terminal peptide bonds of dibasic amino acids.
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- No. CAS: 99676-46-7
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
Actividad biológica
Descripciòn
In Vitro
Protocol
1) Reaction buffer: 50 mM Tris-HCl, 2 mM CaCl2 (pH 7.0-9.0).
2) Storage buffer: 20 mM (pH 5.2) NaAc-HAc, 2 mM CaCl2. If not used immediately after dissolution, the product can be dissolved in storage buffer. The final concentration of the enzyme after dissolution is about 1-10mg/ml, according to It needs to be aliquoted and stored below -20°C.
3) Reaction conditions: The optimal reaction pH is 9.0, and the stable pH is 5.0-6.0.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
3.4.21.61
Specific Activity
≥10 U/mg protein
Unit Definition
One unit is defined as the amount of enzyme that can catalyze substrate Boc-QRR-pNA to release 1 μmol 4-nitroaniline per min, in the 3 mL reaction system of 25°C, 50 mM Tris-HCl, 2 mM CaCl2, pH 8.
Chemical Information
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No. CAS 99676-46-7
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Appearance Solid
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Color White to off-white
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SMILES
[Recombinant Kex2 protease]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
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Ficha de datos (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)