CD24 Protein, Human (HEK293, Fc)

The CD24 protein plays a critical role in cellular differentiation in various cell types, and its signaling may be initiated by lectin-like ligands that bind to the CD24 carbohydrate and transduced by second messengers released from GPI anchors. CD24 also regulates B cell activation responses, promoting AG-dependent B cell proliferation and preventing terminal differentiation into antibody-forming cells. CD24 Protein, Human (HEK293, Fc) is the recombinant human-derived CD24 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD24 Protein, Human (HEK293, Fc) is 33 a.a., with molecular weight of 33-50 kDa.

The CD24 protein is implicated in playing a pivotal role in the cell differentiation of various cell types, with its signaling potentially triggered by the binding of a lectin-like ligand to CD24 carbohydrates, transduced through the release of second messengers derived from the GPI-anchor. CD24 also functions in modulating B-cell activation responses, promoting AG-dependent proliferation of B-cells, and preventing their terminal differentiation into antibody-forming cells. In association with SIGLEC10, CD24 may contribute to the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90, suggesting a role in immune regulation. Additionally, CD24 is implicated in the control of autoimmunity. It exhibits cell-type-specific interactions with LYN and FGR, binding to FRG in a small cell cancer line, LYN in the erythroleukemia cell line K-562, and in Burkitt's lymphoma, highlighting its diverse involvement in cellular processes and immune regulation.

Immobilized Anti-Human CD24 mAb at 2 μg/mL (100 μl/well) can bind Human CD24-Fc*: Biotinylated by NHS-biotin prior to testing The ED₅₀ of Human CD24-Fc* is <7.62 ng/mL.

Human

HEK293

C-hFc

P25063 (S27-G59)

100133941  [NCBI]

SETTTGTSSNSSQSTSNSGLAPNPTNATTKAAG

33-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.