FITC-Labeled VEGFR-2 Protein, Human (HEK293, His)
VEGFR-2 is a tyrosine-protein kinase of the cell surface receptors of VEGFA, VEGFC, and VEGFD that mediates activation of the MAPK1/ERK2, MAPK3/ERK1, and MAP kinase signaling pathways, as well as the AKT1 signaling pathway. VEGFR-2 can promote the proliferation, survival, migration and differentiation of endothelial cells. Overexpression of VEGFR-2 is associated with the development of tumors. FITC-Labeled VEGFR-2 Protein, Human (HEK293, His) is the recombinant human-derived FITC-Labeled VEGFR-2 protein, expressed by HEK293 , with His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 1 year from date of receipt, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
VEGFR-2 is a tyrosine-protein kinase of the cell surface receptors of VEGFA, VEGFC, and VEGFD that mediates activation of the MAPK1/ERK2, MAPK3/ERK1, and MAP kinase signaling pathways, as well as the AKT1 signaling pathway. VEGFR-2 can promote the proliferation, survival, migration and differentiation of endothelial cells. Overexpression of VEGFR-2 is associated with the development of tumors. FITC-Labeled VEGFR-2 Protein, Human (HEK293, His) is the recombinant human-derived FITC-Labeled VEGFR-2 protein, expressed by HEK293 , with His labeled tag.
Background
VEGF receptors (VEGFRs) are receptors for vascular endothelial growth factor (VEGF), and there are three main subtypes, VEGFR-1, VEGFR-2, and VEGFR-3. VEGFR-2 acts as a tyrosine-protein kinase of the cell surface receptor for VEGFA, VEGFC and VEGFD, and plays an important role in angiogenesis, vascular development, vascular permeability and embryonic hematopoiesis. It can promote the proliferation, survival, migration and differentiation of endothelial cells. Promote actin cytoskeleton recombination. VEGFR-2 regulates VEGFR-1 (FLT1) and VEGFR-3 (FLT40) signaling by forming heterodimers. VEGFR-2 mediates the activation of ,MAPK1/ERK2, MAPK3/ERK1 and MAP kinase signaling pathways, as well as the AKT1 signaling pathway. It mediates phosphorylation of the phosphatidylinositol 3-kinase regulatory subunit PIK3R1, recombination of actin cytoskeleton and activation of PTK2/FAK1. Vegfa-mediated induction of NOS2 and NOS3 is required, resulting in endothelial cell production of the signaling molecule nitric oxide (NO). Phosphorylated PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1, and SRC. Overexpression of VEGFR-2 is associated with tumorigenesis[1][2][3][4][5][6].
Technical Parameters
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Species Human
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Source HEK293
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Tag His
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Accession
P35968-1 (A20-E764)
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Gene ID3791
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Protein Length
Extracellular Domain
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Synonyms
KDR; Fetal Liver Kinase 1; Kinase Insert Domain Receptor; Vascular Endothelial Growth Factor Receptor 2 Variant; Vascular Endothelial Growth Factor Receptor 2; Tyrosine Kinase Growth Factor Receptor; VEGFR2; Receptor Protein-Tyrosine Kinase; FLK1; Fetal L
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AA Sequence
ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCRGQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLTIPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSPFIASVSDQHGVVYITENKNKTVVIPCLGSISNLNVSLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISYAGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLSPSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGSGMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGIPLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPISKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGTTQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAVSVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEGKNKTVSTLVIQAANVSALYKCEAVNKVGRGERVISFHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFENLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNATMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKTKKRHCVVRQLTVLERVAPTITGNLENQTTSIGESIEVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGNRNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFIIEGAQEKTNLE
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Predicted Molecular Mass
85.2 kDa
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Molecular Weight
Approximately 110-120 kDa, based on SDS-PAGE under reducing conditions,due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 1 year from date of receipt, protect from light. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
References
[1]. Dougher M, et al. Autophosphorylation of KDR in the kinase domain is required for maximal VEGF-stimulated kinase activity and receptor internalization. Oncogene. 1999 Feb 25;18(8):1619-27. [Content Brief]
[2]. McTigue et al. Crystal structure of the kinase domain of human vascular endothelial growth factor receptor 2: a key enzyme in angiogenesis. Structure. 1999 Mar 15;7(3):319-30. [Content Brief]
[3]. Holmqvist K, et al. The adaptor protein shb binds to tyrosine 1175 in vascular endothelial growth factor (VEGF) receptor-2 and regulates VEGF-dependent cellular migration. J Biol Chem. 2004 May 21;279(21):22267-75. [Content Brief]
[4]. Kroll J, et al. VEGF-A induces expression of eNOS and iNOS in endothelial cells via VEGF receptor-2 (KDR). Biochem Biophys Res Commun. 1998 Nov 27;252(3):743-6. [Content Brief]
[5]. Rho SB, et al. Programmed cell death 6 (PDCD6) inhibits angiogenesis through PI3K/mTOR/p70S6K pathway by interacting of VEGFR-2. Cell Signal. 2012 Jan;24(1):131-9. [Content Brief]
[6]. Guo S, et al. Vascular endothelial growth factor receptor-2 in breast cancer. Biochim Biophys Acta. 2010 Aug;1806(1):108-21. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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