envelope glycoprotein gp120 Protein, HIV-1 (AAA44191, HEK293 , His)
Based on 1 Customer Validation
envelope glycoprotein gp120 Protein is a glycoprotein exposed on the surface of the HIV envelope.Gp120 is essential for viral infection as it facilitates HIV entry into the host cell.gp120 may also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus.envelope glycoprotein gp120 Protein, HIV-1 (AAA44191, HEK293, His) is the recombinant Virus-derived envelope glycoprotein gp120 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Virus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
envelope glycoprotein gp120 Protein is a glycoprotein exposed on the surface of the HIV envelope.Gp120 is essential for viral infection as it facilitates HIV entry into the host cell.gp120 may also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus.envelope glycoprotein gp120 Protein, HIV-1 (AAA44191, HEK293, His) is the recombinant Virus-derived envelope glycoprotein gp120 protein, expressed by HEK293 , with C-10*His labeled tag.
Background
envelope glycoprotein gp120 (gp120) is a glycoprotein exposed on the surface of the HIV envelope. Gp120 is essential for viral infection as it facilitates HIV entry into the host cell and it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and a specific interaction with the CD4 receptor, particularly on helper T-cells. gp120 may also be facilitating viral persistence and continuing HIV infection by influencing the T cell immune response to the virus. Several mechanisms may be involved in this process of which gp120 binds to the CD4 receptor of T cells which facilitates viral entry into the CD4+ cells and their depletion. Gp120 is shed from the viral membrane and accumulates in lymphoid tissues in significant amounts. It can induce apoptosis and severely alter the immune response to the virus by dampening the antiviral CTL response thus impeding the clearance of HIV[1].
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Virus
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Source HEK293
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Tag C-10*His
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Accession
AAA44191.1 (E30-R509)
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Gene ID/
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Molecular Construction
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N-term
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HIV-1 gp120 (E30-R509)
Accession # AAA44191.1 -
10*His
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C-term
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Protein Length
Partial
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Synonyms
Envelope glycoprotein gp160; Env polyprotein; Surface protein gp120; SU; Glycoprotein 120; gp120; Transmembrane protein gp41; TM; Glycoprotein 41; gp41 ; env
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AA Sequence
EEKLWVTVYYGVPVWKEATTTLFCASDRKAYDTEVHNVWATHACVPTDPNPQEVELKNVTENFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLNCTDLRNATNGNDTNTTSSSRGMVGGGEMKNCSFNITTNIRGKVQKEYALFYKLDIAPIDNNSNNRYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGKGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSANFADNAKVIIVQLNESVEINCTRPNNNTRKSIHIGPGRAFYTTGEIIGDIRQAHCNLSRAKWNDTLNKIVIKLREQFGNKTIVFKHSSGGDPEIVTHSFNCGGEFFYCNSTQLFNSTWNVTEESNNTVENNTITLPCRIKQIINMWQEVGRAMYAPPIRGQIRCSSNITGLLLTRDGGPEDNKTEVFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
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Molecular Weight
Approximately 95-150 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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