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  4. HIV-1 gp120
  5. Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His)

Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His)

Cat. No.: HY-P70907
COA Handling Instructions

The envelope glycoprotein gp120 protein facilitates viral capture and dissemination by dendritic cells and endothelial cells by attaching the virus to host lymphocytes through binding to the CD4 receptor. HIV exploits the migratory properties of dendritic cells to access CD4+ T cells, leading to infection. Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His) is the recombinant Virus-derived Envelope glycoprotein gp120 protein, expressed by HEK293 , with C-8*His labeled tag. The total length of Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His) is 498 a.a., with molecular weight of 105-130 kDa.

For research use only. We do not sell to patients.

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Description

The envelope glycoprotein gp120 protein facilitates viral capture and dissemination by dendritic cells and endothelial cells by attaching the virus to host lymphocytes through binding to the CD4 receptor. HIV exploits the migratory properties of dendritic cells to access CD4+ T cells, leading to infection. Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His) is the recombinant Virus-derived Envelope glycoprotein gp120 protein, expressed by HEK293 , with C-8*His labeled tag. The total length of Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His) is 498 a.a., with molecular weight of 105-130 kDa.

Background

Envelope glycoprotein gp160 undergoes oligomerization predominantly into trimers in the host endoplasmic reticulum, followed by transit to the host Golgi for glycosylation completion. Subsequently, gp160 is proteolytically cleaved in the trans-Golgi by cellular furin or furin-like proteases, resulting in the activation and generation of gp120 and gp41. Surface protein gp120 serves as a critical mediator in the viral life cycle, initially attaching the virus to host lymphoid cells through binding to the primary receptor CD4. This interaction induces a structural rearrangement, creating a high-affinity binding site for chemokine coreceptors such as CXCR4 and/or CCR5. Additionally, gp120 acts as a ligand for CD209/DC-SIGN on dendritic cells (DCs) and CLEC4M/DC-SIGNR on endothelial cells, facilitating viral capture at mucosal surfaces and subsequent transmission to permissive cells. This interaction with DCs allows HIV to exploit the migration properties of dendritic cells, gaining access to CD4+ T-cells in lymph nodes. Virus transmission to T-cells can occur in trans (viral capture and transmission) or in cis (following productive DCs infection), contributing to a robust infection. In trans infection, virions bound to DCs remain infectious over days, possibly protected in non-lysosomal acidic organelles within DCs, enhancing viral infectious potential during migration from the periphery to lymphoid tissues. Upon arrival at lymphoid tissues, intact virions recycle back to the DCs' cell surface, facilitating virus transmission to CD4+ T-cells.

Biological Activity

Measured  in a cell proliferation assay using U87 human glioma cell. The  ED50 this effect is 82.60 ng/mL, corresponding to a specific activity  is 1.211×104 units/mg.

Species

Virus

Source

HEK293

Tag

C-8*His

Accession

Q9DKG6 (M1-E498)

Gene ID

/

Molecular Construction
N-term
HIV-1 gp120 (M1-E498)
Accession # Q9DKG6
8*His
C-term
Synonyms
Envelope glycoprotein gp120; Glycoprotein 120; Surface protein gp120
AA Sequence

MRVKETQMNWPNLWKWGTLILGLVIICSASDNLWVTVYYGVPVWRDADTTLFCASDAKAHETEVHNVWATHACVPTDPNPQEIHLENVTENFNMWRNNMVEQMQEDVISLWDQSLKPCVKLTPLCVTLNCTNANWTNSNNTTNGPNKIGNITDEVKNCTFNMTTELKDKKQKVHALFYKLDIVQINSSEYRLINCNTSVIKQACPKISFDPIPIHYCTPAGYAILKCNDKNFNGTGPCKNVSSVQCTHGIKPVVSTQLLLNGSLAEEEIIIRSENLTNNAKTIIVHLNKSVEINCTRPSNNTRTSITMGPGQVFYRTGDIIGDIRKAYCEINGIKWNEVLVQVTGKLKEHFNKTIIFQPPSGGDLEIITHHFSCRGEFFYCNTTKLFNNTCIGNTSMEGCNNTIILPCKIKQIINMWQGVGQAMYAPPISGRINCVSNITGILLTRDGGADNNTTNETFRPGGGNIKDNWRSELYKYKVVEIEPLGIAPTRAKRRVVE

Molecular Weight

105-130 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Envelope glycoprotein gp120 Protein, HIV (Q9DKG6, HEK293, His)
Cat. No.:
HY-P70907
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