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HGFR Protein, Human (sf9, His-GST)

Cat. No.: HY-P72938
COA Handling Instructions

The HGFR protein is a receptor tyrosine kinase that transduces signals by binding to hepatocyte growth factor/HGF ligand. It regulates proliferation, scattering, morphogenesis, and cell survival. HGFR Protein, Human (sf9, His-GST) is the recombinant human-derived HGFR protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.

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2 μg $50 In-stock
10 μg $140 In-stock
20 μg $220 In-stock
50 μg $420 In-stock
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Description

The HGFR protein is a receptor tyrosine kinase that transduces signals by binding to hepatocyte growth factor/HGF ligand. It regulates proliferation, scattering, morphogenesis, and cell survival. HGFR Protein, Human (sf9, His-GST) is the recombinant human-derived HGFR protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

The HGFR protein, a receptor tyrosine kinase, functions as a signal transducer from the extracellular matrix by binding to hepatocyte growth factor/HGF ligand. It plays a pivotal role in regulating diverse physiological processes, including proliferation, scattering, morphogenesis, and cell survival. Upon ligand binding at the cell surface, HGFR undergoes autophosphorylation on its intracellular domain, creating docking sites for downstream signaling molecules. Upon activation by ligand, it interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3, or the adapter GAB1, leading to the activation of multiple signaling cascades, including RAS-ERK, PI3 kinase-AKT, and PLCgamma-PKC. RAS-ERK activation is associated with morphogenetic effects, while PI3K/AKT coordinates prosurvival effects. In embryonic development, HGFR signaling contributes to gastrulation, the development and migration of neuronal precursors, angiogenesis, and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and the proliferation of secondary myoblasts. In adults, it participates in wound healing, organ regeneration, tissue remodeling, and promotes the differentiation and proliferation of hematopoietic cells. Additionally, in the context of microbial infection, HGFR acts as a receptor for Listeria monocytogenes internalin InlB, mediating the entry of the pathogen into cells.

Biological Activity

1. The specific activity was determined to be >10 nmol/min/mg using MBP as substrate.
2. Measured by its binding ability in a functional ELISA. Immobilized human HGFR (aa 956-1390) at 10 μg/mL (100 μl/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/mL.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

P08581-1 (K956-S1390)

Gene ID
Molecular Construction
N-term
His-GST
HGFR (K956-S1390)
Accession # P08581-1
C-term
Synonyms
Hepatocyte growth factor receptor; HGF receptor; SF receptor; MET
AA Sequence

KKRKQIKDLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGSCRQVQYPLTDMSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAEMRPSFSELVSRISAIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSEDNADDEVDTRPASFWETS

Molecular Weight

Approximately 68 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 3 mM DTT

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HGFR Protein, Human (sf9, His-GST)
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HY-P72938
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