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pyrosequencing

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3

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3

Oligonucleotides

Cat. No. Product Name Target Research Areas Chemical Structure
  • HY-185273
    3'-O-(2-Nitrobenzyl)-dGTP

    		DNA/RNA Synthesis Others
    3'-O-(2-Nitrobenzyl)-dGTP is a reversible terminator. 3'-O-(2-Nitrobenzyl)-dGTP can be recognized and incorporated by DNA polymerases, thereby temporarily terminating DNA primer extension; after the 2-nitrobenzyl blocking group is removed via laser irradiation, a free 3'-OH can be regenerated to allow subsequent polymerase-mediated extension. 3'-O-(2-Nitrobenzyl)-dGTP can be used in DNA sequencing studies .
    3'-O-(2-Nitrobenzyl)-dGTP
  • HY-185274
    3'-O-(2-Nitrobenzyl)-dTTP

    		DNA/RNA Synthesis Others
    3'-O-(2-Nitrobenzyl)-dTTP acts as a reversible terminator for DNA chain elongation. 3'-O-(2-Nitrobenzyl)-dTTP interacts with 9°N polymerase (exo-) A485L/Y409V, gets recognized and incorporated into the elongating DNA chain, thereby temporarily terminating the polymerase reaction. 3'-O-(2-Nitrobenzyl)-dTTP carries a 3'-O-(2-nitrobenzyl) blocking group, which can be removed by laser irradiation to regenerate the free 3'-OH − group and restore polymerase activity. 3'-O-(2-Nitrobenzyl)-dTTP is applicable in DNA sequencing studies .
    3'-O-(2-Nitrobenzyl)-dTTP
  • HY-185272
    3'-O-(2-Nitrobenzyl)-dCTP

    		DNA/RNA Synthesis Others
    3'-O-(2-Nitrobenzyl)-dCTP is a reversible terminator. 3'-O-(2-Nitrobenzyl)-dCTP can be recognized and incorporated by polymerases, thereby temporarily terminating primer extension during DNA synthesis. After incorporation into the extending DNA strand, 3'-O-(2-Nitrobenzyl)-dCTP temporarily halts the DNA polymerase extension reaction, and its 3'-O-(2-nitrobenzyl) blocking group can be removed by laser irradiation to regenerate a free 3'-OH for subsequent primer extension cycles. 3'-O-(2-Nitrobenzyl)-dCTP is applicable for resolving homopolymeric regions in DNA templates .
    3'-O-(2-Nitrobenzyl)-dCTP

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