SK48-E26 

SK48-E26 is a human interleukin-1β (IL-1β) inhibitor, with a IC₅₀ of 400 pM against human IL-1β, a IC₅₀ of 185 pM against cynomolgus monkey IL-1β, and a K_a value ranging from 0.048 nM to 3 nM for human IL-1β. SK48-E26 binds to the conformational epitope of human IL-1β spanning amino acid residues 95-101, and blocks the biological activity of IL-1β. SK48-E26 can be used in research related to IL-1β-mediated inflammatory diseases and osteoarthritis^[1][2].

Human

SK48-E26 effectively captures human recombinant IL-1β in an ELISA format, enabling detection of hrIL-1β at concentrations as low as 0.1 to 0.2 ng/mL with a linear response to IL-1β concentration^[1].
SK48-E26 specifically neutralizes human recombinant IL-1β activity in an EL-4 cell-based bioassay, binds a conformational epitope on hrIL-1β spanning amino acids 95-101, and has an affinity for IL-1β of 0.5 to 3 nM^[1].
Mutations in the heavy chain CDR3 region of SK48-E26 Fab fragments increase affinity for human recombinant IL-1β, with the V120T/R121K mutant showing a 9.79-fold improvement over the wild-type SK48-E26 Fab^[1].
SK48-E26 (10-200 nM) binds to human IL-1β with an equilibrium dissociation constant of 242 pM^[2].
SK48-E26 (1×10^-15-1×10^-7 M; 1 h pre-incubation with IL-1β, 16-20 h incubation with cells) neutralizes human IL-1β with an IC₅₀ of 400 pM and cynomolgus IL-1β with an IC₅₀ of 185 pM in MRC-5 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3553  [NCBI]

P01584

IL-1b

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

[SK48-E26]

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Peter Ronald Young, et al. RECOMBINANT AND HUMANIZED IL-1β ANTIBODIES FOR TREATMENT OF IL-1 MEDIATED INFLAMMATORY DISORDERS IN MAN. WO1995001997A1. 1994.

  [2]. Lacy SE, et al. Generation and characterization of ABT-981, a dual variable domain immunoglobulin (DVD-Ig(TM)) molecule that specifically and potently neutralizes both IL-1α and IL-1β. MAbs. 2015;7(3):605-19.  [Content Brief]