TCO-PEG5-C2-Mal
Based on 1 Customer Validation
TCO-PEG5-maleimide is a PEG linker with TCO and maleimide groups, specifically enabling tetrazine click chemistry and thiol conjugation for bioconjugation, probe design, and drug delivery.
For research use only. We do not sell to patients.
- Purity : 95.0%
- CAS No.: 2748951-17-7
- Formula: C25H40N2O9
- Molecular Weight:512.59
-
Storage:
Solution, -20°C, 2 years
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
-
Help & FAQs
Biological Activity
Description
In Vitro
TCO-PEG5-C2-Mal is a heterobifunctional linker that can be used for tetrazine ligation and sulfhydryl conjugation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
-
CAS No. 2748951-17-7
-
Appearance Liquid
-
Molecular Weight 512.59
-
Formula C25H40N2O9
-
Color Colorless to light yellow
-
SMILES
O=C(OC1CC/C=C/CCC1)NCCOCCOCCOCCOCCOCCN2C(C=CC2=O)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Solution, -20°C, 2 years
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
-
Data Sheet (236 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
TCO-PEG5-C2-Mal Related Classifications
HY-164785 Related Classifications
Powered by Bioz